The substitution of His 64 of carbonic anhydrase II with Ala results in a sharp decrease in the activity of the enzyme in HEPES buffer (molecular weight of HEPES = 238.3 g/mol). However, increasing concentrations of imidazole (molecular weight = 68.1 g/mol) restores the reaction rate close to that of the wild-type enzyme. Propose an explanation for these results.
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The substitution of His 64 of carbonic anhydrase II with Ala results in a sharp decrease in the activity of the enzyme in HEPES buffer (molecular weight of HEPES = 238.3 g/mol). However, increasing concentrations of imidazole (molecular weight = 68.1 g/mol) restores the reaction rate close to that of the wild-type enzyme. Propose an explanation for these results.
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