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This is a homework question that I have on my Adv. Anatomy & Physiology assignment. How to I got about answering the question? (I do have the Codon table in my textbook)Consider a hypothetical protein that consists of the amino acid sequence arginine, phenylalanine and histidine.  From this information, construct (1) the original DNA strand, (2) corresponding mRNA codons and (3) tRNA anticodons used to produce this hypothetical protein.  (Only use 1 mRNA codon per amino acid.)  In addition, be sure to include start and stop sequences where appropriate.

Question

This is a homework question that I have on my Adv. Anatomy & Physiology assignment. How to I got about answering the question? (I do have the Codon table in my textbook)

Consider a hypothetical protein that consists of the amino acid sequence arginine, phenylalanine and histidine.  From this information, construct (1) the original DNA strand, (2) corresponding mRNA codons and (3) tRNA anticodons used to produce this hypothetical protein.  (Only use 1 mRNA codon per amino acid.)  In addition, be sure to include start and stop sequences where appropriate.

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Step 1

The hypothetical protein considered here consists of the amino acid sequence arginine, phenylalanine, and histidine. The abbreviations for these amino acids are Arg, Phe, and His respectively. There are 20 amino acids which are coded by 64 codons. A codon is a sequence of three DNA or RNA nucleotides that corresponds with a specific amino acid or stop signal during protein synthesis. Out of the possible 64 codons, there are three such codons, namely UGA, UAA, UAG, which do not code for any amino acid but marks the end of protein synthesis. As there are 64 codons for 20 amino acids, it is understood that one protein is coded by more than one codon in most of the cases. Also, there is a start codon, AUG, which marks the initiation of the translation process.

Step 2

Arg, Phe, and His are coded by CGU, UUU, and CAU respectively. As we also need to include a start and a stop codon, let us take AUG as the start codon and UAG as the stop codon. Hence, the mRNA sequence for the given amino acid sequence would be as follows:

Amino acid sequence
Arg Phe His
mRNA
AUG CGU UUU CAU UAG
Here, AUG is the start codon and UAG is the stop codon
CGU codes for Arg, UUU codes for Phe, and CAU codes for His
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Amino acid sequence Arg Phe His mRNA AUG CGU UUU CAU UAG Here, AUG is the start codon and UAG is the stop codon CGU codes for Arg, UUU codes for Phe, and CAU codes for His

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Step 3

The tRNA, by complementary base pairing, wo...

Amino acid sequence
Arg Phe His
mRNA
AUG CGU UUU CAU UAG
tRNA anticodon
UAC GCA AAA GUA AUC
help_outline

Image Transcriptionclose

Amino acid sequence Arg Phe His mRNA AUG CGU UUU CAU UAG tRNA anticodon UAC GCA AAA GUA AUC

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