Q: please answer Why is it useful to have two different mechanisms of positive regulation? I.e. why…
A: The L-arabinose operon, also called the ara or araBAD operon, is an operon required for the…
Q: Predict a possible phenotype from the following mutations in the Lac Operon: a deletion in…
A: Lac operon was discovered by Jacob and Monod in Escherichia coli. They found that this bacteria…
Q: Another name for the RNA polymerase recognition/binding site upstream of a gene is the O a. promoter…
A: Introduction : In transcription process RNA polymerase binds to the DNA upstream of the gene at a…
Q: Expansion of a CAG repeat region by 1 repeat is an example of a frameshift mutation. Both DNA…
A: Frameshift mutation: It is a kind of genetic disorder that occurs due to insertion or deletion of…
Q: Identify a Transcription Factor (TF) / DNA Regulatory Protein (DRP) that is functional in Humans…
A: Answer: Introduction: Eukaryotic cellular process is regulated in the transcription process and RNA…
Q: Compare and contrast the lac operon and trp operon of E. coli.
A: E.coli is a type of bacteria that is mostly found in the human intestine and gut of some animals. It…
Q: Choose correct option and do explain plz. 1. The protein complex that helps RNA polymerase to…
A: a) SWI/SNF It is composed of various proteins and act as an ATP-dependent chromatin remodeling…
Q: Compare and contrast the lac operon and repressible operon systems.
A: Expression of each and every gene is not always required in an organism. Gene expression must occur…
Q: Predict the effect on the activity of the lac operon of a mutation that disrupts the function of: a)…
A: The Catabolic Activator Protein (CAP) is a protein which acts as a positive regulating factor for…
Q: Describe what happens to the trp operon as the cell uses up its store of tryptophan.
A: The cells have amino acids in various forms such as precursors or native form. Amino acids are an…
Q: How does the Trp operon work? A. Binding of Trp to a repressor protein allows the repressor to bind…
A: Trp operon is the cluster of genes that are responsible for synthesis pf operon
Q: Operons are clusters of genes that are regulated as a group. They can be characterized as either…
A: Operon Operon is the collection or group of structural genes that is controlled by one promoter.…
Q: What would be the effect on tryptophan production of mutations in region 1 of the leader of the trp…
A: Step 1 In operon like tryptophan operon transcription starts from transcription start site, but get…
Q: that best fits the phrase below (1-20) Cis-acting B Post-transcriptional modification…
A: Francis Crick proposed central dogma. The RNA is formed from DNA with the help of enzyme RNA…
Q: Operons______ . a. only occur in bacteria b. include multiple genes c. involve selective gene…
A: The correct answer is (b) include multiple genes
Q: Explain how the lac operon is regulated when both glucose and lactose is present. Make sure you…
A: An operon is a unit of gene expression that is present in bacteria and it helps to regulate gene…
Q: Prokaryotes are lacking. in their DNA sequence. A. introns B. exons. O C. promoter sites D. operons
A: Prokaryotes are the primitive type of cells that don't have membrane bound organelles and have the…
Q: 83) The RDRP of the rotavirus a. Only produced in the first round of translation b. It is…
A: RdRP- is RNA dependent RNA polymerase. All retroviruses have RNA dependent RNA polymerases.
Q: Prokaryotes use operons to regulate their transcripts. What does it mean for them to be…
A: Prokaryotes are a simpler form of life. It lacks sophisticated machinery and membrane-bound…
Q: Examining the DNA of a cancerous cell, a scientist would find strong expression of A. siRNAs. B.…
A: Option c i.e. oncogenes is the correct statement for the above question.
Q: The trp operon is a repressible operon system. What does this mean?
A: Trp refers to tryptophan. The operon is the functional unit of Deoxyribonucleic acid ( DNA) having…
Q: A nonsense mutation Select one: a. results in a larger protein being produced from the mutated gene…
A: Introduction :- There are a number of disorders known to be caused by nonsense mutations, including:…
Q: Attached is a region of DNA containing a transcription unit. Match the boxes with the terms…
A: The DNA contained the genetic information that is transcribed into mRNA by the process of…
Q: If the levels of both Glucose and Lactose were low in a bacterial cell, describe the state of the…
A: In bacteria and their viruses, an operon is a genetic regulatory structure in which genes coding for…
Q: Which statement about the transcription attenuation mechanism is TRUE? 1.In some operons (e.g., the…
A: Transcriptional attenuation is a regulatory mechanism that causes premature termination of…
Q: 2. You are studying the regulation of the lactose operon in Escherichia coli, by measuring…
A: The lac operon is responsible for the entry and metabolism of lactose in E. coli as well as most…
Q: What is lac operon system?
A: Lac operon is one of the method by which a prokaryotic cell control gene expression. The lac operon…
Q: Defend or attack this statement: “All eukaryotic promoters have TATA boxes.”
A: Promoter is defined as DNA region where initiation of gene transcription takes place. They regulate…
Q: In the trp operon, tryptophan is best characterized as being a: A. repressor B. inducer C.…
A: Operon is clustered of Genes where a single mRNA consists of more than one functional gene.It is…
Q: RNA polymerase binds to a specific region known as the _? OAUG Exon 1 Transcription Start Site…
A: RNA polymerase is an enzyme that assists in the transcription process by transcribing a DNA sequence…
Q: Why do eukaryotic cells not have a lac operon? A. Eukaryotic cells cannot transcribe multiple genes…
A: Lac operon is a gene regulating unit present in prokaryotes. It consists of a promoter, repressor,…
Q: when tryptophan is present, what would happen to trp operon regulation
A: Introduction:- The trp operon is a collection of genes identified in E. coli bacteria that code for…
Q: In Tetrahymena, the removal of the single intron in the rRNA precursor does not involve a protein. O…
A: Central dogma includes three steps of transfer of genetic information which are replication,…
Q: I. Answer the following: 1. What are the main elements of the lac operon and their functions? 2.…
A: Definition:- An operon is a group of genes that are transcribed at the same time. These are a…
Q: You are studying a new operon that you have discovered in E. Coli that you have named the Late…
A:
Q: In the lac and trp operons, the repressors are said to be "allosteric molecules" because they…
A: Operons are regions present in DNA which are composed of related genes.Operons are generally found…
Q: Determine the advantage of regulating the binding of RNA polymerase to DNA at the promoter.
A: The synthesis of RNAs and proteins in accordance with the instructions contained in deoxyribonucleic…
Q: Some transcription factors need to bind to the DNA directly and others to the enzyme to recruit it…
A: This question is based on transcription process in the cell.
Q: The heptapeptide repeat in the carboxy terminal domain (CTD) in the RPB1 subunit of RNA polymerase…
A: Answer :- Option (D) is correct. - Initiate transcription.
Q: Tryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too…
A: The trp Operon, is nothing but a sequence of DNA that is responsible for the regulation of formation…
Q: Complete the sentences below using the words and phrases on the left. Then arrange the text boxes to…
A: 1) DNA double helix, open promoter complex 2) Transcription mRNA, hairpin loop 3) Sigma factor
Q: High levels of glucose in the environment result in low levels of transcription of the lac operon.…
A: The lactose operon (lac operon) is an operon needed for the transport and digestion of lactose in…
Q: Binding of the prokaryotic DNA dependent RNA polymerase to promoter site is inhibited bya)…
A: in order to address the q, we'll first analyse the function of each of the antibiotic in the options…
Q: in a western blot how come changes in mrna levels don't always match the protein product of that…
A: The western blot:It is also known as the protein immunoblot. It is an extensively used analytical…
Q: Matching type Choices are in the picture 11. regulating elements in the operon 12. ribosome…
A: Transcription is the process by which the information in a strand of DNA is copied into a new…
Q: Explain the steps in Trp Operon. Take note, important enzymes and chemicals are needed in your…
A: The trp operon is a group of genes that codes for the molecules that are responsible for the…
Q: In the txp operon, a repressor protein acquires a mutation such that it cannot bind to tryptophan.…
A: BASIC INFORMATION TRYPTOPHAN OPERON This system is known as Repressible Operon System. It can also…
Q: Operons are clusters of genes that are regulated as a group. They can be characterized as either…
A: the correct answer is binding to a repressor molecule turns repressible operons off and inducible…
Q: In the ara operon in E. coli cells, AraC will bind to its specific target DNA when O a. when there…
A: AraC protein can shift from the state of its preference to loop DNA (when arabinose is absent) to…
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- 46What is the A-site of the ribosome? A.exit siteb.aminoacyl-tRNA binding sitec.peptidyl-tRNA binding siteD.peptidyltransferase site 47This sequence motif, which is called the ( ) , is usually located 25 to 30 bp upstream from the transcription initiation site. 48Pre-mRNA requires specific sequences for precise __________ to occur. A.splicingb.taggingc.replicationD.skippingPost-transcriptional Modification of Eukaryotic mRNAs What might be the advantages of capping, methylation, and polyadenylylation of eukaryotic mRNAs?A fragment of bacterial DNA reads: 3’ -TACCTATAATCTCAATTGATAGAAGCACTCTAC- 5’ Assuming that this fragment is the template strand, what is the sequence of mRNA that would he transcribed? (Hint: Be sure to identify the initiation site.)
- Substrate Binding by RNA Polymerase RNA polymerase has t binding sites for ribonucleoside triphosphates: the initiation site and the elongation site. The initiation site has a greater Km for NTPs than the elongation site. Suggest what possible significance this fact might have for the control of transcription in cells.The Second Genetic Code Review the evidence establishing that aminoacyl-tRNA synthetases bridge the information gap between amino acids and codons. Indicate the various levels of specificity possessed by aminoacyl-tRNA synthetases that are essential for high-fidelity translation of messenger RNA molecules.Transcription AttenuationHow would transcriptionof the E. coli trp operon be affected by the following manipulations of the leader region of the trp mRNA?(a) Increasing the distance (number of bases) betweenthe leader peptide gene and sequence 2(b) Increasing the distance between sequences 2 and 3(c) Removing sequence 4(d) Changing the two Trp codons in the leader peptidegene to His codons(e) Eliminating the ribosome-binding site for the genethat encodes the leader peptide(f) Changing several nucleotides in sequence 3 so thatit can base-pair with sequence 4 but not with sequence 2
- 88Sequential binding of RNA polymerase II-TFIIF complex, TFIIE, and TFIIH completes ___________________ formation. A.pre-initiation complexb.TF recognition elementc.pre-elongation complexD.TATA binding complex 89Which of the following is the GDP-GTP exchange protein?A.EF-Tub.none of the abovec.EF-TsD.EF-G 90RNA polymerase II has 14 subunits. Yesorno28Elongation factor __________ reactivates arrested RNA polymerase II. A.TFIIEb.TFIISc.TFIIFD.TFIID 29Eukaryotic translation initiation proceeds through a ( ) mechanism. 30A variety of transcription ______________ factors helps to suppress transient pausing during elongation. A.terminationb.elongationc.none of the aboveD.initiationPlease help with all parts of A, B, C, D 2. You are studying the function of a messenger RNA named Genetixrox and want to label themRNA with a radioactive atom. Assume the mRNA is long and contains all four standardRNA bases. Assume that the cell cannot convert ribonucleotides to deoxyribonucleotides (orvice versa).A. Will you generate radioactive Genetixrox mRNA with 3H-threonine? Threonine is an aminoacid. Answer yes or no, and provide a one sentence rationale.B. Will you generate radioactive Genetixrox mRNA with 3H-adenosine triphosphate? Answeryes or no, and provide a one sentence rationale.C. Will you generate radioactive Genetixrox mRNA with 3H-deoxyadenosine triphosphate?Answer yes or no, and provide a one sentence rationale.D. Will you generate radioactive Genetixrox mRNA 12C-with adenosine triphosphate? Answeryes or no, and provide a one sentence rationale
- Trinucleotide repeat expansions (TNREs) are associated with severaldifferent human inherited diseases. Certain types of TNREsproduce a long stretch of the amino acid glutamine within theencoded protein. When a TNRE exerts its detrimental effect byproducing a glutamine stretch, are the following statements true orfalse?A. The TNRE is within the coding sequence of the gene.B. The TNRE prevents RNA polymerase from transcribing thegene properly.C. The trinucleotide sequence is CAG.D. The trinucleotide sequence is CCG.26. differential lengths of poly-A tails affect mRNA stability identify which describes the statement above a. pre-transcriptional controlb. transcriptional controlc. translational controld. post-translational control v) A normal mRNA that reads 5'- UGCCAUGGUAAUAACACAUGAAGGCCUGAAC-3' was an insertion mutation that changes the sequence to 5'- UGCCAUGGUUAAUAACACAUGAGGCGUGAAC-3'. Translate the original mRNA and the mutated mRNA and explain how insertion mutations can have dramatic effects on proteins. ( Hint; Be sure to find the initiation site).
