How would transcription of the E. coli trp operon be affected by the following manip ulations of the leader region of the trp mRNA?

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter30: Protein Synthesis
Section: Chapter Questions
Problem 12P
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How would transcription
of the E. coli trp operon be affected by the following manip
ulations of the leader region of the trp mRNA?
(a) Increasing the distance (number of bases) between
the leader peptide gene and sequence 2
(b) Increasing the distance between sequences 2 and 3
(c) Removing sequence 4
(d) Changing the two Trp codons in the leader peptide
gene to His codons
(e) Eliminating the ribosome-binding site for the gene
that encodes the leader peptide
(f) Changing several nucleotides in sequence 3 so that
it can base-pair with sequence 4 but not with sequence 2

 

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