Concepts of Genetics (12th Edition)
Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 11, Problem 26ESP
Summary Introduction

To design: An experiment to establish the fact that DNA replication is conservative.

Introduction: Taylor, Woods, and Hughes experiment demonstrated the semiconservative mode of DNA (deoxyribonucleic acid) replication in the root tips of plant Vicia faba.

Summary Introduction

To draw: Sister chromatids and demonstrate the expected results of conservative mode of DNA replication.

Introduction: The process of replication can be monitored by labeling DNA with 3H-thymidine using autoradiography. It is a common technique that pinpoints the location of a radioisotope in a cell.

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What are the three models of DNA replication? With the aid of illustrations, show how the Meselson Stahl experiment come to the conclusion of one model of DNA replication. Is DNA replication bidirectional? How did you arrive at this conclusion? Explain the bacterial replication model that supports this conclusion.
You conducted an experiment to determine the mechanism of DNA replication in the hypothetical organism Fungus mungus. Your data shows that synthesis of  newly replicated DNA from F. mungus is discontinuous on both strands of the replication fork. Does this result support or not support the hypothesis that F. mungus replicates its DNA by the same mechanism as yeast? Briefly explain your answer.
Using 14N isotope medium for DNA replication instead of 15N, what would be observed ifDNA replication were conservative in one cycle of replication/in three cycles? How aboutdispersive replication in one cycle, in three cycles?

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Concepts of Genetics (12th Edition)

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Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license