Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 11, Problem 13PDQ
List and describe the function of the ten subunits constituting DNA polymerase III. Distinguish between the holoenzyme and the core enzyme.
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List and describe the function of the ten subunits constituting DNA polymerase III. Distinguish between the holoenzyme and the core enzyme.
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Does the addition of a histidine tag affect DNA polymerase activity and or processivity? Give a detailed explanation.
Chapter 11 Solutions
Concepts of Genetics (12th Edition)
Ch. 11 - In the Meselson-Stahl experiment, which of the...Ch. 11 - An alien organism was investigated. When DNA...Ch. 11 - Why might mutations in genes encoding telomerase...Ch. 11 - Although the brother is an immunologically matched...Ch. 11 - Prob. 3CSCh. 11 - HOW DOWE KNOW? In this chapter, we focused on how...Ch. 11 - CONCEPT QUESTION Review the Chapter Concepts list...Ch. 11 - Compare conservative, semiconservative, and...Ch. 11 - Describe the role of 15N in the MeselsonStahl...Ch. 11 - Predict the results of the experiment by Taylor,...
Ch. 11 - What are the requirements for in vitro synthesis...Ch. 11 - In Kornbergs initial experiments, it was rumored...Ch. 11 - How did Kornberg assess the fidelity of DNA...Ch. 11 - Which characteristics of DNA polymerase I raised...Ch. 11 - Kornberg showed that nucleotides are added to the...Ch. 11 - What was the significance of the polA1 mutation?Ch. 11 - Summarize and compare the properties of DNA...Ch. 11 - List and describe the function of the ten subunits...Ch. 11 - Distinguish between (a) unidirectional and...Ch. 11 - List the proteins that unwind DNA during in vivo...Ch. 11 - Define and indicate the significance of (a)...Ch. 11 - Outline the current model for DNA synthesis.Ch. 11 - Why is DNA synthesis expected to be more complex...Ch. 11 - Suppose that E. coli synthesizes DNA at a rate of...Ch. 11 - Several temperature-sensitive mutant strains of E....Ch. 11 - While many commonly used antibiotics interfere...Ch. 11 - Describe the end-replication problem in...Ch. 11 - Many of the gene products involved in DNA...Ch. 11 - In 1994, telomerase activity was discovered in...Ch. 11 - The genome of D. melanogaster consists of...Ch. 11 - Prob. 26ESPCh. 11 - DNA polymerases in all organisms add only 5...Ch. 11 - Assume that the sequence of bases shown below is...Ch. 11 - Reiji and Tuneko Okazaki conducted a now classic...Ch. 11 - Consider the drawing of a dinucleotide below. (a)...Ch. 11 - To gauge the fidelity of DNA synthesis, Arthur...
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- Provide a brief summary of the Sanger sequencing method.arrow_forwardDescribe the function of DNA polymerase. Explain why each part of the name DNA polymerase (DNA, polymer, -ase) makes sense.arrow_forwardIn one, simple sentence define the function of the following 1. Helicase = 2. Alpha subunit of DNA polymerase III =arrow_forward
- Considering prokaryotes, what is the enzyme that helps hold DNA polymerase III in place when nucleotides are being added?arrow_forwardDescribe how DNA gyrase causes DNA supercoiling.arrow_forwardName and describe the first four events (and associated enzymes) that happen during DNA replication at the DNA double helix (in correct sequence)arrow_forward
- With regard to DNA replication, define the term bidirectional replication.arrow_forwardExplain the concept & importance of DNA polymerase III ?arrow_forwardSummarize the functions of the following proteins in E. coli DNA replication: DNA polymerase I, DNA polymerase III, DnaA, helicase, SSB, primase, the sliding clamp, clamp loader, DNA ligase, Tus, and topoisomerases.arrow_forward
- Name of a few enzymes involved in DNA replication other than DSNA polymerase and ligase.name the key function each of them?arrow_forwardWhat is an Okazaki fragment? In which strand of replicating DNA are Okazaki fragments found? Based on the properties of DNA polymerase, why is it necessary to make these fragments?arrow_forwardAssume that a cycle of the polymerase chain reaction takes 20 minutes. How many copies of a single DNA fragment will there be after the reaction has been run for six hours?arrow_forward
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