Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Question
Chapter 11, Problem 39RQ
Summary Introduction
To review:
The following processes, and compare them by providing a suitable example:
a) Primary vs (versus) secondary active transport.
b) Passive vs facilitated diffusion.
c) Carrier-mediated vs channel-mediated transport.
Introduction:
The cell of living beings has a special feature of transporting the
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Chapter 11 Solutions
Biochemistry: The Molecular Basis of Life
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- The following question focuses on how the parameters regulating enzyme function might change, and how these might appear graphically on a Michaelis-Menten plot and a Lineweaver-Burke plot. Carbonic anhydrase is an enzyme that will convert CO2 and water into HCO3. CO2 + H20 > H+ + HCO3 There are many different isoforms of this enzyme. (see for instance http://en.wikipedia.org/wiki/Carbonic_anhydrase 1 Assume that one variant has a Km of 10 µM and a different variant has a Km of 100 µM. Draw on the same graph a typical Michaelis-Menton plot showing the alteration in the rate of carbonic anhydrase as the CO2 level is varied for the two different variants of enzyme, assuming the concentration of the enzyme (10 mM) in the test tube is kept constant. Assume that you have equal amounts of the two different variants of carbonic anhydrase in a number of test tubes and that the Vmax for both enzymes are the same. Be sure to label the axes. For the same conditions as above, draw a…arrow_forwardThe transverse diffusion of lipids is thermodynamically unfavorable because of the difficulty of its hydrophilic head to pass through an internal hydrophobic environment. True Falsearrow_forwardWhat do you mean by Golgi-resident enzymes?arrow_forward
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