BIO 102 General Biology II Updated Edition (Tidewater Community College)
3rd Edition
ISBN: 9781259614064
Author: Tidewater Community College
Publisher: MCG CUSTOM
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Question
Chapter 11, Problem 3WIO
Summary Introduction
To explain:
How ingredients in a PCR reaction tube replicate DNA.
Concept introduction:
PCR stands for Polymerase Chain Reaction. It is a technique used in molecular biology to amplify sequence of DNA and produce several copies of DNA. This technique was developed by Kary Mullis.
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Chapter 11 Solutions
BIO 102 General Biology II Updated Edition (Tidewater Community College)
Ch. 11.1 - Prob. 1MCCh. 11.1 - Prob. 2MCCh. 11.2 - What are some uses for transgenic organisms?Ch. 11.2 - Prob. 2MCCh. 11.2 - Prob. 3MCCh. 11.2 - What is the function of the 98.5% of the human...Ch. 11.2 - How does PCR work, and why is it useful?Ch. 11.2 - Prob. 6MCCh. 11.2 - Prob. 7MCCh. 11.3 - Prob. 1MC
Ch. 11.3 - Prob. 2MCCh. 11.3 - Summarize the steps scientists use to clone an...Ch. 11.3 - Prob. 4MCCh. 11.4 - Prob. 1MCCh. 11.4 - Prob. 2MCCh. 11.4 - Prob. 3MCCh. 11.4 - What are some examples of ethical questions raised...Ch. 11.5 - Prob. 1MCCh. 11.5 - Prob. 2MCCh. 11 - If a restriction enzyme cuts between the G and the...Ch. 11 - Prob. 2MCQCh. 11 - Prob. 3MCQCh. 11 - Prob. 4MCQCh. 11 - Prob. 5MCQCh. 11 - Preimplantation genetic diagnosis would be least...Ch. 11 - Prob. 7MCQCh. 11 - What techniques might researchers use to create...Ch. 11 - Prob. 2WIOCh. 11 - Prob. 3WIOCh. 11 - Prob. 4WIOCh. 11 - Prob. 5WIOCh. 11 - Prob. 6WIOCh. 11 - Unneeded genes in an adult animal cell are...Ch. 11 - 8. Search the Internet for examples of mammals...Ch. 11 - Prob. 9WIOCh. 11 - Prob. 10WIOCh. 11 - If a cells genome is analogous to a cookbook and a...Ch. 11 - Prob. 1PITCh. 11 - Add the terms restriction enzyme, plasmid, virus,...Ch. 11 - Prob. 3PIT
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardDescribe the method of a PCR technique in which you can amplify fragments randomly? Briefly.arrow_forwardDescribe what occurs in each of the three steps during the PCR reaction.arrow_forward
- Briefly describe the functions of the components of a typical PCR reaction.arrow_forwardExplain the concepts of excitation and emission in fluorescence in qRT-PCRarrow_forwardExplain the role of each of the two enzymes used in the room temperature -PCR process. Reverse transcriptas Taq DNA polymerasearrow_forward
- Explain the different steps of a PCR reaction completely and in detailarrow_forwardLook at each PCR component listed below. For each one, determine which steps(s) of the PCR reaction (denaturation, annealing or extension) would be directly affect if that component were missing. Taq polymerase: Oligonucleotide primers: DNA template: Deoxynucleotides (A, T, G and C): Imagine that you correctly prepare your PCR reaction mixture, but there is something wrong with the thermal cycler. Describe what would happen if: The thermal cycler was stuck on 940C: The thermal cycler cycled between 600C and 720C, but never reached 940C The thermal cycler cycled between 940C and 720C, but never reached 600arrow_forwardPolymerase Chain Reaction (PCR) works by exposing the reaction to a series of temperature changes. In 150 words or fewer, describe 1.) the different stages of a single PCR cycle, 2.) how many cycles of PCR your reactions will undergo, and 3.) why multiple cycles are necessary.arrow_forward
- Which is a complete list of the ingredients that are essential for PCR? nucleotides, DNA template, Taq polymerase, and plasmids nucleotides, DNA template, DNA ligase, and plasmids nucleotides, DNA template, Taq polymerase, and primers restriction enzymes, DNA template, Taq polymerase, and primers nucleotides, DNA template, DNA ligase, and primersarrow_forwardDuring DNA isolation, you incubated the tissue sample at 55°C for 60 minutes, followed by incubation at 95°C for 10 minutes. What happens to the DNA molecules in this last 95°C incubation? What would happen to the PCR if this step was omitted?arrow_forwardQuantitative PCR differs from regular PCR in that it uses [A] to [B] the amount of [C] in a sample. It cannot quantify [D] unless it is first made into [F]. Match each of the following to its appropriate letter: quantify, cDNA, RNA, DNA or RNA, fluorescence. 1) A 2) B 3) C 4) D 5) E Here are the choices for the questions a) quantify b) RNA c) cDNA d) fluorescence e) DNA or RNAarrow_forward
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