Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Textbook Question
Chapter 11, Problem 6TY
Meselson and Stahl were able to demonstrate semiconservative replication in E. coli by
- a. using radioactive isotopes of phosphorus to label the original strand and visually determining the relationship of original and new DNA strands.
- b. using different enzymes to eliminate old strands from DNA.
- c. using isotopes of nitrogen to label the DNA and determining the relationship of original and new DNA strands by density differences of the new DNA molecules.
- d. labeling viral DNA before it was incorporated into a bacterial cell and visually determining the location of the DNA after centrifugation.
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Consider the experiment conducted by Meselson and Stahl in which they used 14N and 15N in cultures of E. coli and equilibrium density gradient centrifugation. Draw pictures to represent the bands produced by bacterial DNA in the centrifuge tube before the switch to medium containing 14N and after one, two, and three rounds of replication in that medium. Use separate sets of drawings to show the bands that would appear if replication were (a) semiconservative; (b) conservative; (c) dispersive.
Describe the Meselon -Stahl experiment Include how they distinguished new from old DNA strands, what the centrifuge was for, and how it showed semi-conservative replication.
The Meselson and Stahl experiment used a density label toa. determine the directionality of DNA replication.b. differentially label DNA and protein.c. distinguish between newly replicated and old strands.d. distinguish between replicated DNA and RNA primers.
Chapter 11 Solutions
Biology
Ch. 11.1 - Prob. 1CSCh. 11.1 - Prob. 1EQCh. 11.1 - Prob. 2EQCh. 11.1 - CoreSKILL In the experiment of Avery, MacLeod,...Ch. 11.2 - Prob. 1CCCh. 11.2 - Prob. 2CCCh. 11.2 - Core Skill: Modeling The goal of this modeling...Ch. 11.2 - Prob. 2CSCh. 11.3 - If this experiment was conducted for four rounds...Ch. 11.4 - Molecular Mechanism of DNA Replication Concept...
Ch. 11.4 - Prob. 2CCCh. 11.4 - Prob. 3CCCh. 11.4 - Prob. 4CCCh. 11.5 - Prob. 1CCCh. 11.5 - Prob. 1CSCh. 11 - Why did researchers initially believe that the...Ch. 11 - Prob. 2TYCh. 11 - Which of the following equations is accurate...Ch. 11 - Prob. 4TYCh. 11 - Which of the following statements about the...Ch. 11 - Meselson and Stahl were able to demonstrate...Ch. 11 - During replication of a DNA molecule, the daughter...Ch. 11 - Prob. 8TYCh. 11 - A nucleosome is a. a dark-staining body composed...Ch. 11 - The conversion of euchromatin into heterochromatin...Ch. 11 - What are the four key criteria that the genetic...Ch. 11 - A double-stranded DNA molecule contains 560...Ch. 11 - Prob. 3CQCh. 11 - Prob. 1COQCh. 11 - CoreSKILL How might you provide evidence that DNA...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Would it be possible to start synthesizing the daughter DNA strand without assembling the RNA primer first? Why? Why not? A. Yes, because the 3' OH is already present in the DNA strand which will be used as a template. B. No, because the RNA primer which contains the free 5' PO4 in its ribose will not be synthesized by primase. C. Yes, because the 5' PO4 is already present in the DNA strand which will be used as a template. D. No, because the RNA primer which contains the free 3' OH in its ribose has to be synthesized by primase first.arrow_forwardIn the Meselson-Stahl experiment, what happened after the E. coli was moved to the N14 medium and two rounds of cell division occurred? This result demonstrated that DNA replicated in a semi-conservative manner. A. The DNA formed one band at the N15 density level and one band at the N14 density level. B. The DNA formed one band between the N14 and N15 density levels and one band at the N14 density level. .C. The DNA only formed one band between the N14 and N15 density levels.arrow_forwardCompare and contrast the properties of the enzymes DNA polymerase I and polymerase III from E. coli.arrow_forward
- a. Explain whether or not DNA polymerase from a mesophilic bacterium could be used successfully in a PCR reaction. b. If starting with a single double-stranded DNA molecule, how many copies of the DNA would be synthesized after 25 PCR cycles?arrow_forwardCompare DNA polymerase and RNA polymerase from E. coli in regard to each of the following features: (a) activated precursors,(b) direction of chain elongation, (c) conservation of the template, and(d) need for a primer.arrow_forwardDid Hershey and Chase overcome objections to Avery et al.’s claim that DNA, not protein, was the genetic material? How was the choice of a bacteriophage by Hershey and Chase a way to avoid problems with earlier bacterial transformation experiments?arrow_forward
- DNA Polymerase holoenzymes used for DNA replication recognizes A. double-stranded sequences as starting points B. methylated lipids as start points C. acetylated lipids as start points D. single stranded sequences as starting pointsarrow_forwardHow did the results prove the semiconservative model of DNA replication? Explainarrow_forwardA. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the plasmid with Xhol and Xbal, which lane of the agarose gel represents the DNA fragments you would expect from the digestion? B. If you now decide to cut the plasmid with EcoRI, how many fragments will be produced and what will their sizes be? C. When running DNA samples on agarose gel, an electric field is applied. Towards which electrode will the DNA migrate and why?arrow_forward
- During PCR amplification in preparation for DNA sequencing, why were there different colors at the 3’ ends of the fragments produced? (What did these four colors represent?)arrow_forwardWhen joining two or more DNA fragments, a researcher can adjust the sequence at the junction in a variety of subtle ways, as seen in the following exercises.(a) Draw the structure of each end of a linear DNA fragment produced by an EcoRI restriction digest (include those sequences remaining from the EcoRI recognition sequence).(b) Draw the structure resulting from the reaction of this end sequence with DNA polymerase I and the four deoxynucleoside triphosphates.(c) Draw the sequence produced at the junction that arises if two ends with the structure derived in (b) are ligated (d) Draw the structure produced if the structure derived in (a) is treated with a nuclease that degrades only single-stranded DNA.(e) Draw the sequence of the junction produced if an end with structure (b) is ligated to an end with structure (d).(f) Draw the structure of the end of a linear DNA fragment that was produced by a PvuII restriction digest (include those sequences remaining from the PvuII recognition…arrow_forwardA.How could endonucleases interfere with the transformation procedure? B. Does supercoiled or nicked plasmid get transformed more efficiently? Why?arrow_forward
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