Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
expand_more
expand_more
format_list_bulleted
Question
Chapter 12, Problem 17E
Summary Introduction
To explain: The way in which diisopropylphosphofluoridate (DIPF) affects the apparent KM and Vmax of a sample of chymotrypsin.
Concept introduction: KM is the Michaelis-Menten constant. Vmax is the maximum velocity with which the enzyme catalyzes the reaction. Diisopropylphosphofluoridate (DIPF) reacts only with chymotrypsin (Ser 195 amino acid residue) that helped to identify the active site Ser of the enzyme chymotrypsin.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionChapter 12 Solutions
Fundamentals of Biochemistry: Life at the Molecular Level
Ch. 12 - Prob. 1ECh. 12 - Prob. 2ECh. 12 - Prob. 3ECh. 12 - Prob. 4ECh. 12 - 5. From the reaction data below, determine whether...Ch. 12 - Prob. 6ECh. 12 - 7. For an enzymatic reaction, draw curves that...Ch. 12 - 8. Explain why it is usually easier to calculate...Ch. 12 - Prob. 9ECh. 12 - 10. Identify the enzymes in Table 12-1 whose...
Ch. 12 - 11. Calculate KM and Vmax from the following...Ch. 12 - 12. Explain why each of the following data sets...Ch. 12 - 13. The KM for the reaction of chymotrypsin with...Ch. 12 - 14. Enzyme A catalyzes the reaction S ? P and has...Ch. 12 - 15. In a bisubstrute reaction, a small amount of...Ch. 12 - 16. In a bisubstrate reaction, a small amount of...Ch. 12 - Prob. 17ECh. 12 - 18. Molecule A is the substrate for enzyme X....Ch. 12 - 19. Determine the type of inhibition of an...Ch. 12 - 20. Estimate KI for a competitive inhibitor when...Ch. 12 - Prob. 21CQCh. 12 - 22 Is it necessary to know [E]T to determine (a)...Ch. 12 - 23. You are trying to determine the KM for an...Ch. 12 - 24. You are attempting to determine KM by...Ch. 12 - Prob. 25CQCh. 12 - 26. Enzyme X and enzyme Y catalyze the same...Ch. 12 - 27. Based on some preliminary measurements, you...Ch. 12 - 28. For the same enzyme sample described in...Ch. 12 - 29. For an enzyme catalyzed reaction, the presence...Ch. 12 - 30. Sphingosine-1-phosphate (S1P) is important for...Ch. 12 - 31. Ethanol in the body is oxidized to...Ch. 12 - Prob. 32CQCh. 12 - MORE TO EXPLORE What kind of inhibitor is...
Knowledge Booster
Recommended textbooks for you
- BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. FreemanLehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. FreemanFundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningFundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON
Biochemistry
Biochemistry
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:9781464126116
Author:David L. Nelson, Michael M. Cox
Publisher:W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul...
Biochemistry
ISBN:9781118918401
Author:Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:WILEY
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Fundamentals of General, Organic, and Biological ...
Biochemistry
ISBN:9780134015187
Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:PEARSON