Concept explainers
(a)
To explain: The type of inhibition when 5nM of reversible inhibitor yields a Vmax of 80% of value in the absence of the inhibitor and the KM value is unchanged.
Concept introduction: Enzymes are biological protein catalysts which alter the
(b)
To explain: The proportion of enzyme molecules bound to the inhibitor when 5nM of reversible inhibitor yields a Vmax of 80% of value in the absence of the inhibitor.
Concept introduction: Enzymes are biological protein catalysts which alter the speed of the reaction in the biological system. Enzyme inhibitors are those that block the enzyme activity. KM is the Michaelis-Menton constant. KM value is not a direct measure of the affinity of the enzyme towards the substrate, but the concentration of S, where the velocity at maximum is half. Vmax is the maximum velocity which the enzyme catalyzes the reaction.
(c)
To calculate: Inhibition constant
Concept introduction: Enzymes are biological protein catalysts which alter the speed of the reaction in the biological system. Enzyme inhibitors are those that block the enzyme activity. KM is the Michaelis-Menton constant. KM value is not a direct measure of the affinity of the enzyme towards the substrate, but the concentration of S, where the velocity at maximum is half. Vmax is the maximum velocity which the enzyme catalyzes the reaction.
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