GENETIC ANALYSIS: INTEGRATED - ACCESS
GENETIC ANALYSIS: INTEGRATED - ACCESS
3rd Edition
ISBN: 9780135349298
Author: Sanders
Publisher: PEARSON
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Chapter 12, Problem 22P

Suppose the lac operon partial diploid cap - I + P + O + Z - Y + /cap + I - P + O + Z + Y - is grown.

a. Will this partial diploid strain grow on a lactose medium?

b. Is transcription of β- galactosidase and permease inducible, constitutive, or noninducible?

c. Explain how genetic complementation contributes to the growth habit of this strain.

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Suppose you have six strains of E. coli. One is wildtype, and each of the other five has a single one of thefollowing mutations: lacZ−, lacY−, lacI−, oc, andlacIS. For each of these six strains, describe thephenotype you would observe using the following assays. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expressionbut cannot serve as a carbon source for bacterialgrowth because it cannot be cleaved byβ-galactosidase; (2) X-gal cannot serve as a carbonsource for growth; (3) E. coli requires active lactosepermease (the product of lacY) to allow lactose,X-gal, or IPTG into the cells.] Colony color in medium containing glycerol as theonly carbon source and X-gal, but no IPTG.d. Colony color in medium containing high levels ofglucose as the only carbon source, X-gal, andIPTG.e. Colony color in medium containing high levels ofglucose as the only carbon source and X-gal, butno IPTG
Give all possible genotypes of a lac operon that produces, or fails to produce, B-galactosidase and permease under the following conditions. Do not give partial-diploid genotypes. Lactose present B-Galactosidase Lactose absent B-Galactosidase Permease Permease a. b. C. d. e. f. g. + I + II + + + | +I
The map of the lac operon is shown below. Consider the following examples that include both haploids and partial diploids and explain in each scenario whether the repressor can bind and regulate expression and whether or not the lac operon is expressed. For partial diploids the plasmid is indicated by the F’. I+ O+ Z+ Y+ / F’ I+ O+ Z+ Y+   I- O+ Z+ Y+ / F’ I+ O+ Z+ Y+   I- O+ Z+ Y+    I+ Oc Z+ Y+

Chapter 12 Solutions

GENETIC ANALYSIS: INTEGRATED - ACCESS

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