A protein molecule is discovered in mice and that can cure cancer. The protein is present in tiny amount. What steps would be used to produce this protein molecule in therapeutic amounts. Which host would be used to clone the gene and why. Which host would be used to express this protein and why. Concept introduction: The mice genome contains both exons (coding regions) and introns (non-coding regions). Hence, before cloning the DNA sequence must be modified (contain only exons). The expression vector is used to carry the gene of interest into the host for gene expression.

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Chapter 12, Problem 3AQ

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A protein molecule is discovered in mice and that can cure cancer. The protein is present in tiny amount. What steps would be used to produce this protein molecule in therapeutic amounts. Which host would be used to clone the gene and why. Which host would be used to express this protein and why.

Concept introduction:

The mice genome contains both exons (coding regions) and introns (non-coding regions). Hence, before cloning the DNA sequence must be modified (contain only exons). The expression vector is used to carry the gene of interest into the host for gene expression.

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