Chapter 12, Problem 6CONQ

Summary Introduction

To review:

To determine the positions of -35 sequence, which are tolerant to change and their importance in binding of σ (sigma) factor.

Introduction:

A consensus sequence is a sequence of DNA having similar structure and function in different organisms. A shorter consensus sequence is found in TATA box. It is termed so because of the presence of repeated T (thymine) and A (adenine) base pairs. In bacteria, two consensus sequences have been found, namely a sequence at the -35 position ($\text{5’-TTGACA-3’}$) and a sequence at the -10 position ($\text{5- TATAAT-3’}$).