EBK BROCK BIOLOGY OF MICROORGANISMS
15th Edition
ISBN: 8220103633352
Author: Stahl
Publisher: PEARSON
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Chapter 12.6, Problem 1CR
Summary Introduction
Genetic engineering uses microorganisms to produce several essential products for human needs such as human hormones, enzymes, drugs, chemicals, and fuels. It is a science of
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Research has now demonstrated that techniques that have been developed for cloning animals could be used to clone human embryos, although no living human clones have been produced. There is widespread consensus that cloning a live human would be unethical. However, some people have suggested creating cloned embryos from which stem or pluripotent cells, which have the ability to develop into any tissue or organ, could be extracted. The stem and pluripotent cells might then be used to create genetically identical tissues and organs that could then be transplanted back into the donor to treat failed organs and diseases. What might be some reasons for or against this type of therapeutic cloning?
a piece of DNA is produced with
1) an enhancer from a gene expressed in the liver,
2) a promoter from a gene expressed in bone cells, and
3) exons and introns from a gene expressed in the brain
introduction of this gene into a mouse then results in high levels of
A) a brain protein in the liver
B) a bone protein in the brain
C) a brain protein in the bone
D) a liver protein in the brain
E) no protein would be made
Chapter 12 Solutions
EBK BROCK BIOLOGY OF MICROORGANISMS
Ch. 12.1 - Why is a primer needed at each end of the DNA...Ch. 12.1 - How does RT-PCR differ from traditional PCR?Ch. 12.1 - Prob. 3MQCh. 12.1 - Describe the basic principles of gene...Ch. 12.2 - What is the purpose of molecular cloning?Ch. 12.2 - Prob. 2MQCh. 12.2 - Prob. 3MQCh. 12.2 - Prob. 1CRCh. 12.3 - How can the bacteriophage T7 promoter be used to...Ch. 12.3 - What major advantage does cloning mammalian genes...
Ch. 12.3 - Prob. 3MQCh. 12.3 - Prob. 1CRCh. 12.4 - How can site-directed mutagenesis be useful to...Ch. 12.4 - What is used to alter more than a few base pairs...Ch. 12.4 - What are knockout mutations?Ch. 12.4 - What does site-directed mutagenesis allow you to...Ch. 12.5 - What is a reporter gene? The product of which...Ch. 12.5 - Prob. 2MQCh. 12.5 - Describe two widely used reporter genes.Ch. 12.6 - Prob. 1MQCh. 12.6 - Prob. 2MQCh. 12.6 - Prob. 3MQCh. 12.6 - Prob. 1CRCh. 12.7 - Prob. 1MQCh. 12.7 - Give an example of a genetically modified plant...Ch. 12.7 - How have transgenic salmon been engineered to...Ch. 12.7 - What is the Ti plasmid and how has it been of use...Ch. 12.8 - Explain why recombinant vaccines might be safer...Ch. 12.8 - Prob. 2MQCh. 12.8 - Prob. 3MQCh. 12.8 - What is a subunit vaccine and why are subunit...Ch. 12.9 - Explain why metagenomic cloning gives large...Ch. 12.9 - What types of environments are often sampled to...Ch. 12.9 - Prob. 3MQCh. 12.9 - How has metagenomics been used to find novel...Ch. 12.10 - How has Caldicellulosiruptor been modified to...Ch. 12.10 - Prob. 2MQCh. 12.10 - What has been the limiting factor in engineering...Ch. 12.10 - Prob. 1CRCh. 12.11 - What are biobricks?Ch. 12.11 - Prob. 2MQCh. 12.11 - How was Escherichia coli modified to produce a...Ch. 12.11 - Prob. 1CRCh. 12.12 - Prob. 1MQCh. 12.12 - Prob. 2MQCh. 12.12 - How is recombinant DNA inserted into a genome...Ch. 12.12 - How has the CRISPR editing technology been applied...Ch. 12.13 - Prob. 1MQCh. 12.13 - How can a tRNA be engineered to encode for a...Ch. 12.13 - Prob. 3MQCh. 12.13 - What are some mechanisms for controlling a...Ch. 12 - Suppose you have just determined the DNA base...Ch. 12 - Prob. 2AQCh. 12 - Prob. 3AQCh. 12 - Describe how you could recode Escherichia coli to...
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- You just graduated from college and started working at a biotech startup called Scrofabulous. Your first job assignment is to clone the pig gene for the hormone prolactin. Assume that the pig gene for prolactin has not yet been isolated, sequenced, or mapped; What would be the most useful and economical first step to go about identifying and cloning the pig gene for prolactin? use the amino acid sequence of mouse prolactin to design a pair of degenerate oligonucleotide PCR primers to PCR-amplify the pig prolactin gene. RNAseq the pituitary gland of the pig, the most abundant gene is likely to to be prolactin Conduct a proteome search for peptides that match parts of mouse prolactin protein Sequence the pig genome, then translate the genome to find the gene predicted to encode for prolactin Crystalize the mouse prolactin protein and use Google's DeepMind Al to find the closest amino acid sequence in the pig proteomearrow_forwardBriefly discuss the following concerns of Biotechnology;(i) Monoclonal antibodies technology versus gene technology for cancer treatment.(ii) Traditional plant breeding versus Gene technology: Which is the more efficient way for pesticide resistant crop production?(iii) Chemical technology versus Enzyme technology in industrial application.(iv) Are genetically modified organisms (GMOs) a threat to the environment?arrow_forward1. Two closely related species may have a very similar genome size but very different number of a)nucleosomes B)mitochondrial chromosomes C)nucleoli D)chromosomes 2. What is required for RNA polymerase to be able to dissociate from transcription factors and start transcribing the DNA? A)acetylation of histones B)methylation of the DNA that will be transcribed C)phosphorylation of the RNA polymerase tail D)phosphorylation of transcription factorsarrow_forward
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