Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780321962751
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 13, Problem 7TYU
Summary Introduction
Introduction:
The semi-conservation model of
The synthesis of the other strands will take place during DNA replication to form the intermediate bands in the first generation. In the second generation of replication, one low density band and one intermediate density band will be formed.
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A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe?
a. The reaction will work, but at a significantly slower rate.
b. The reaction will work, but the product will contain many undesired mutations.
c. The reaction will work, but amplify a region that was not his target.
d. The reaction will be completely unsuccessfu
(A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the desired amplification product?
(B) What about after 5 cycles. Assume that we started with one molecule in each case, and that the reaction is perfectly efficient.
The given figure shows the denaturation curves for three DNA samples.
A) What is the ?m of the most GC-rich DNA sample?
B) Which point represents double-stranded DNA?
Chapter 13 Solutions
Campbell Biology in Focus (2nd Edition)
Ch. 13.1 - Given a polynucleotide sequence such as GAATTC,...Ch. 13.1 - Prob. 2CCCh. 13.2 - What role does base pairing play in the...Ch. 13.2 - Make a table listing the functions of seven...Ch. 13.2 - MAKE CONNECTIONS What is the relationship between...Ch. 13.3 - Describe the structure of a nucleosome, the basic...Ch. 13.3 - What two properties, one structural and one...Ch. 13.4 - Prob. 1CCCh. 13.4 - DRAW IT One strand of a DNA molecule has the...Ch. 13.4 - Describe the role of complementary base pairing...
Ch. 13 - In his work with pneumonia-causing bacteria and...Ch. 13 - What is the basis for the difference in how the...Ch. 13 - In analyzing the number of different bases in a...Ch. 13 - The elongation of the leading strand during DNA...Ch. 13 - Prob. 5TYUCh. 13 - Prob. 6TYUCh. 13 - Prob. 7TYUCh. 13 - Prob. 8TYUCh. 13 - Prob. 9TYUCh. 13 - MAKE CONNECTIONS Although the proteins that cause...Ch. 13 - Prob. 12TYUCh. 13 - FOCUS ON EVOLUTION Some bacteria may be able to...Ch. 13 - FOCUS ON ORGANIZATION The continuity of life is...
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- DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.arrow_forwardYou used agarose gel electrophoresis to separate DNA fragments of different size and the experiment worked well. However, you wanted to re run the experiment but this time you made the gel with a higher percentage of Agarose. How might this affect your results compared to the first run? a. There would be no difference between the runs since it is the current, not the agarose that causes migration. b. The higher concentration of agarose would cause the DNA to break apart. c. You can't predict how the concetration of agarose would affect migration. d. The DNA fragments would migrate further down the gel than they did the first time. e. The DNA fragments wouldn't migrate as far down the gel as they did the first time.arrow_forwardIn the Meselson-Stahl experiment, what happened after the E. coli was moved to the N14 medium and two rounds of cell division occurred? This result demonstrated that DNA replicated in a semi-conservative manner. A. The DNA formed one band at the N15 density level and one band at the N14 density level. B. The DNA formed one band between the N14 and N15 density levels and one band at the N14 density level. .C. The DNA only formed one band between the N14 and N15 density levels.arrow_forward
- ARE THEY TRUE OR FALSE? a) In a caesium chloride density gradient centrifugation method performed in the presence of ethidium bromide, supercoiled plasmid DNA binds more ethidium bromide than linear DNA. B)Tth DNA polymerase shows DNA-dependent DNA polymerase activity as well as RNA-dependent DNA polymerase activity. C)Magnesium ions stimulate polymerase activity. Therefore, it is better to use the highest concentration of magnesium in PCR amplification. D)When lambda bacteriophage infects E. coli cell lysis never occurs, and the infected bacterium can continue to grow and divide. E)The copy number refers to the number of molecules of an individual plasmid that are normally found in a single bacterial cell. F)RNase H selectively hydrolyzes phosphodiester bonds of RNA molecules in RNA:DNA duplexes.arrow_forwardTo prepare a sample for electrophoresis, samples of the DNA being investigated (a) are put into each of four tubes and induced to replicate (b). Also, into the first tube, an adenine terminator was added in addition to all the other nucleotides. As the complementary strand was being constructed, the terminators were occasionally incorporated wherever an adenine nucleotide was used. This random incorporation resulted in all possible lengths of DNA pieces that had an adenine on the end (c). The same process was conducted in the other tubes with thymine, guanine and cytosine terminators; one treatment for each of the four lanes in the gel. Electrophoresis separated the replicated pieces of DNA by size. Staining the gel revealed which lengths of the complementary DNA were terminated by which nucleotide terminators (d). The gel consists of four “lanes,” labeled A, T, G, and C, indicating either adenine, thymine, guanine, or cytosine terminated pieces of DNA. By “reading” down the gel, you…arrow_forwardIf you have G-C rich DNA versus A-T rich DNA a.Will the melting temperatures be the same/ Why? b.Will the annealing temperature be the same? c. Which of these categories is prone to have more errors?arrow_forward
- Describe the possible outcome of a PCR experiment in which (a) there is a single-stranded break in the target DNA sequence, which is present in only one copy in the starting sample, and (b) there is a doublestranded break in the target DNA sequence, which is present in only one copy in the starting sample.arrow_forwardIn a melting profile for DNA, the absorbance at 260 nm increases as result of the disruption of the DNA structure. What is the fundamental physical basis for the absorbance increase? a. the effective concentration of DNA changes b. the absorbance of DNA is highly sensitive to temperature c. native DNA absorbs light more strongly than denatured DNA because the bases are stacked d. denatured DNA absorbs light more strongly than native DNA because the bases have become unstackedarrow_forwardE. coli cells grown on 15N medium are transferred to 14N mediumand allowed to grow for two more generations (two rounds ofDNA replication). DNA extracted from these cells is centrifuged.What density distribution of DNA would you expect in thisexperiment?(A) one high-density and one low-density band(B) one intermediate-density band(C) one high-density and one intermediate-density band(D) one low-density and one intermediate-density bandarrow_forward
- In isolating DNA in strawberries, can you isolate other biomolecules aside from DNA just by using commonly available household materials and chemicals? Elaborate on your answer.arrow_forwarda. This piece of DNA is cut by EcoRI, the resulting fragments are separated by gel electrophoresis, and the gel is stained with ethidium bromide. Draw a picture of the bands that will appear on the gel. b. If a mutation that alters EcoRI site 1 occurs in this piece of DNA, how will the banding pattern on the gel differ from the one that you drew in part a? c. If mutations that alter EcoRI sites 1 and 2 occur in this piece of DNA, how will the banding pattern on the gel differ from the one that you drew in part a? d. If 1000 bp of DNA were inserted between the two restriction sites, how would the banding pattern on the gel differ from the one that you drew in part a? e. If 500 bp of DNA between the two restriction sites were deleted, how would the banding pattern on the gel differ from the one that you drew in part a?arrow_forwardIn experiments using polymerase chain reactions (PCR), it is often more difficult to amplifythrough regions of DNA that are high in GC content versus those regions that are either lower inGC content or are AT-rich. Based on your knowledge of DNA structure, explain why.arrow_forward
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