Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780321962751
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 13, Problem 8TYU
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Introduction:
A student worked in the laboratory and conducted an experiment on the
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In the dideoxy-sequencing reaction, what terminates DNA synthesis at a particular base? a. The absence of a base on the ddNTP halts the DNA polymerase. b. The ddNTP causes a break in the sugar–phosphate backbone. c. DNA polymerase will not incorporate a ddNTP into the growing DNA strand. d. The absence of a 3′-OH group on the ddNTP prevents the addition of another nucleotide.
Which statement about Okazaki fragments is true?
Select one:
a. DNA polymerase doesn’t need a primer to build these fragments
b. They act as a primer that initiates DNA replication.
c. They correct errors made during earlier phases of DNA replication.
d. They are necessary because DNA polymerase can only build DNA in the 5’ to 3’ direction, so for one of the strands at each fork, the DNA polymerase can only buildaway from the fork.
e. They prevent the ends of chromosomes from shortening with every replication.
What is the function of resolvase in recombination?
a. It unwinds double-stranded DNA.
b. It allows a single DNA strand to invade a DNA helix.
c. It displaces one of the original DNA strands during branch migration.
d. It cleaves the Holliday structure.
Chapter 13 Solutions
Campbell Biology in Focus (2nd Edition)
Ch. 13.1 - Given a polynucleotide sequence such as GAATTC,...Ch. 13.1 - Prob. 2CCCh. 13.2 - What role does base pairing play in the...Ch. 13.2 - Make a table listing the functions of seven...Ch. 13.2 - MAKE CONNECTIONS What is the relationship between...Ch. 13.3 - Describe the structure of a nucleosome, the basic...Ch. 13.3 - What two properties, one structural and one...Ch. 13.4 - Prob. 1CCCh. 13.4 - DRAW IT One strand of a DNA molecule has the...Ch. 13.4 - Describe the role of complementary base pairing...
Ch. 13 - In his work with pneumonia-causing bacteria and...Ch. 13 - What is the basis for the difference in how the...Ch. 13 - In analyzing the number of different bases in a...Ch. 13 - The elongation of the leading strand during DNA...Ch. 13 - Prob. 5TYUCh. 13 - Prob. 6TYUCh. 13 - Prob. 7TYUCh. 13 - Prob. 8TYUCh. 13 - Prob. 9TYUCh. 13 - MAKE CONNECTIONS Although the proteins that cause...Ch. 13 - Prob. 12TYUCh. 13 - FOCUS ON EVOLUTION Some bacteria may be able to...Ch. 13 - FOCUS ON ORGANIZATION The continuity of life is...
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- (a) What is the function of helicase in DNA replication?(b) What is the function of DNA polymerase?(c) What are replication forks? Compare and contrast leading and lagging strands. Answer all pleasearrow_forwardA biochemist isolates, purifies and combines ina test tube a variety of molecules needed for DNA replication. When she adds some DNA to the mixture, replication occurs, but each DNA molecule consists of a normal strand paired with numerous segments of DNA a few hundred nucleotides long. What has she probably left out of the mixture? a.DNA polymerase b. DNA ligase c. Okajaki fragments d. primasearrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forward
- The following diagram represents a DNA molecule that is undergoing replication. Draw in the strands of newly synthesized DNA and identify (a) the polarity of the newly synthesized strands, (b) the leading and lagging strands, (c) Okazaki fragments, and (d) RNA primers.arrow_forwardSuccessful bacterial DNA replication does NOT … please explain the answer choice a.ligate DNA nicks b.use a bidirectional fork c.prime new synthesis using DNA primers d.have SSB (single strand binding protein) stabilize single stranded DNAarrow_forwardBase analogs are mutagenic because of which characteristic? a. They produce changes in DNA polymerase that cause it to malfunction. b. They distort the structure of DNA. c. They are similar in structure to the normal bases. d. They chemically modify the normal bases.arrow_forward
- DNA polymerase III is a processive enzyme, which means that a. it does not dissociate from the growing strand after it has attached a nucleotide to the 3′ end. b. it makes a new strand very quickly. c. it proceeds toward the opening of the replication fork. d. it copies DNA with relatively few errors.arrow_forwardFrom which end of a strand of nucleic acid does DNA polymerase I REMOVE nucleotides? A) 5' B) 3' C) 2' D) N-term E) C-termarrow_forwardWhat is the function of Ligase during DNA Replication a Releases tension on the DNA as it unwinds b Puts down RNA primers so replication can continue c Binds Okazaki Fragments into a continuous DNA strand d Binds to single stranded DNA to protect it from degradationarrow_forward
- A scientist uses a molecule of DNA composed of radioactive nucleotides as a template for replication and transcription in a nonradioactive environment. a) What percentage of individual strands of DNA will be radioactive after three (3) DNA replication cycles? 2. b) What percentage of RNA will be radioactive?arrow_forwardThe Meselson and Stahl experiment used a density label toa. determine the directionality of DNA replication.b. differentially label DNA and protein.c. distinguish between newly replicated and old strands.d. distinguish between replicated DNA and RNA primers.arrow_forwardDNA replication is said to be semiconservative because a. one of the new molecules conserves both of the original DNA strands. b. the new DNA molecule contains two new DNA strands. c. both of the new molecules contain one new strand and one old strand. d. DNA polymerase conserves both of the old strands.arrow_forward
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