Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 13, Problem 9P
Compare and contrast the transcriptional regulation ofGAL genes in yeast with that of the lac genes in bacteria.
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Briefly explain How does post-transcriptional regulation work in prokaryotic and euk
Based on the information in Figure 12-6, how doesGal4 regulate four different GAL genes at the sametime? Contrast this mechanism with how the Lac repressor controls the expression of three genes.
Name the lambda promoters whose expression is regulated by the cro protein. For each promoter you named, is cro an activator or a repressor of transcription from that promoter?
Chapter 13 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 13 - 13.1 Devoting a few sentences to each, describes...Ch. 13 - 13.2 Describe and give an example (real or...Ch. 13 - What is meant by the term chromatin remodeling?...Ch. 13 - 13.4 What general role does acetylation of histone...Ch. 13 - 13.5 Describe the roles of writers, readers, and...Ch. 13 - Outline the roles of RNA in eukaryotic gene...Ch. 13 - 13.7 What are the roles of the Polycomb and...Ch. 13 - Most biologists argue that the regulation of gene...Ch. 13 - Compare and contrast the transcriptional...Ch. 13 - The term heterochromatin refers to heavily...
Ch. 13 - 13.11 Compare and contrast promoters and enhancers...Ch. 13 - 13.12 What are the different chromatin...Ch. 13 - 13.13 Define epigenetics, and provide examples...Ch. 13 - What is one proposed role for lncRNAs?Ch. 13 - 13.15 What are the sources of dsRNA? Diagram the...Ch. 13 - How does dsRNA lead to posttranscriptional gene...Ch. 13 - 13.17 A hereditary disease is inherited as an...Ch. 13 - Prob. 18PCh. 13 - Prob. 19PCh. 13 - 13.20 A muscle enzyme called ME is produced by...Ch. 13 - Using the components in the accompanying diagram,...Ch. 13 - 13.22 The majority of this chapter focused on gene...
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- Explain how the data shown indicate that two operator sites are necessary for repression of the lac operon. What would the results have been if all three operator sites were required for the binding of lac repressor?arrow_forwardDiagram the lactose operon in E. coli with the properorder of the elements lacI, lacO, lacY, and lacZ, and explainhow expression is controlled in the presence and in theabsence of lactose.arrow_forwardA crucial step in the regulation of many bacterial genes is the binding of RNA polymerase to DNA at the promoter. Why might it be advantageous for bacteria to regulate the expression of their genes at this particular step?arrow_forward
- Explain why RNAi would be a less effi cient mechanism for regulating the expression of specifi c genes if Dicer hydrolyzed double-stranded RNA every 11 bp rather than every 22 bp.arrow_forwardThe PIC is highly conserved among species, from yeast to humans. Why is the positioning of the first TFII protein so important for proper transcription of the gene?arrow_forwardExplain how the following mutations would affect transcription of the yeast GAL1 gene in the presence of galactose. (a) A deletion within the GAL4 gene that removes the region encoding amino acids 1 to 100. (b) A deletion of the entire GAL3 gene. (c) A mutation within the GAL80 gene that blocks the ability of Gal80 protein to interact with Gal3p. (d) A deletion of one of the four UASG elements upstream from the GAL1 gene. (e) A point mutation in the GAL1 core promoter that alters the sequence of the TATA box.arrow_forward
- The lac repressor and the pur repressor are homologous proteins with very similar three-dimensional structures, yet they have different effects on gene expression. Describe two important ways in which the gene-regulatory properties of these proteins differ.arrow_forwardGenes in both prokaryotes and eukaryotes are regulated by activators and repressors.a. Compare and contrast the mechanism of functionof a prokaryotic repressor (for example, Lac repressor) with a typical eukaryotic repressor protein(a direct repressor).b. Compare and contrast the mechanism of functionof a prokaryotic activator (for example, CAP) witha typical eukaryotic activator protein.arrow_forwardExplain why inactivation of the O1 sequence of the lac operator almost completely abolishes repression of the lac operon.arrow_forward
- Describe the regulation of expression of the lac Z, Y and A genes in E.coli when there is LOW glucose and HIGH lactose present in the cell.arrow_forwardExplain how HOTAIR plays a role in the transcriptional regulationof particular genes.arrow_forwardThe yeast gene SER3, whose product has a role in serine biosynthesis, is repressed during growth in nutrient-rich medium, so little transcription takes place, and little SER3 enzyme is produced, under these conditions. In an investigation of the repression of the SER3 gene, a region of DNA upstream of SER3 was found to be heavily transcribed when SER3 is repressed ). Within this upstream region is a promoter that stimulates the transcription of an RNA molecule called SRG1 RNA (for SER3 regulatory gene 1). This RNA molecule has none of the sequences necessary for translation. Mutations in the promoter for SRG1 result in the disappearance of SRG1 RNA, and these mutations remove the repression of SER3. When RNA polymerase binds to the SRG1 promoter, the polymerase travels downstream, transcribing the SGR1 RNA, and passes through and transcribes the promoter for SER3. This activity leads to the repression of SER3. Propose a possible explanation for how the transcription of SGR1 might…arrow_forward
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