CAMPBELL BIOLOGY IN FOCUS (LL)-W/MOD.MA
CAMPBELL BIOLOGY IN FOCUS (LL)-W/MOD.MA
3rd Edition
ISBN: 9780135686065
Author: Urry
Publisher: PEARSON
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Chapter 13.4, Problem 1CC
Summary Introduction

To identify:

The sequence and the types of the bonds that are being cleaved in the sequence of the DNA by the restriction enzyme PvuI namely:

5'-CGATCG-3'3'-GCTAGC-5'

Concept introduction:

The enzyme PvuI cuts in between the T-C nucleotide in the sequence given. The sequence after the digestion of the restriction enzyme sequence will be:

5'-CGAT--CG-3'3'-GC--TAGC-5'

The types of the bonds that will be cleaved in between the nucleotides are the phosphate bonds between the pentose sugar and the nucleotide connecting the next nucleotide in the sequence.

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MAKE CONNECTIONS The restriction site for an enzymecalled PvuI is the following sequence:5’-CGATCG-3’3’-GCTAGC-5’
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