Concept explainers
a. Calculate the standard free energy change as a pair of electrons is transferred from succinate to molecular oxygen in the mitochondrial respiratory chain.
b. Based on your answer in part a, calculate the maximum number of protons that could be pumped out of the matrix into the intermembrane space as these electrons are passed to oxygen. Assume 25o C, ΔpH = 1.4;
c. At which site(s) are these protons pumped?
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- Assume that in a certain cell, the ratio of products/reactants or Keg = 809.5 (Keq is dimensionless) for the reaction Glucose + 2ATP > Glucose-1,6-diP + 2ADP, at a particular instant, the concentrations of each compound were Glucose =2.4M, ATP =11.1M, ADP -12.8M and G-6-P -28.4M. Calculate the difference (dimensionless) between Keq and the ratio of products/ractants at this instance, in this cell, to five decimal placesarrow_forwardImagine that in red blood cells (RBCS) the following concentrations were noted for the "adenylate pool": [ATP] = 2000 uM; [ADP] = 270 uM; and [AMP] = 30 uM. ASsume that the pH is 7 and remains constant. a) calculate the Energy change (EC) (to four decimal places for RBCS given the above values. Show work b) If the [ATP] decreases by 5%, calculate the new [ATP], [ADP], and [AMP] given that the concentrations of all three species are "connected" by the adenylate kinase reaction, which is shown below. You will need to use the quadratic equation for this one. show your work. %3| %3D ADP + ADP ATP + AMP Keq = 0.44arrow_forward.Intramitochondrial ATP concentrations are about 5 mM, and phosphate con- centration is about 10 mM. If ADP is five times more abundant than AMP, calculate the molar concentrations of ADP and AMP at an energy charge of 0.85. Calculate AG for ATP hydrolysis at 37 °C under these conditions. The energy charge is the concentration of ATP plus half the concentration of ADP divided by the total adenine nucleotide concentration: [ATP] + 1/2[ADP] [ATP] + [ADP] + [AMP]arrow_forward
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