Concept explainers
(a)
Interpretation:
The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.
Concept introduction:
The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or
(b)
Interpretation:
The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.
Concept introduction:
The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.
(c)
Interpretation:
The significance of specific binding attaining a plateau should be determined.
Concept introduction:
The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.
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