Concept explainers
(a)
Interpretation:
The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.
Concept introduction:
The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or
(b)
Interpretation:
The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.
Concept introduction:
The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.
(c)
Interpretation:
The significance of specific binding attaining a plateau should be determined.
Concept introduction:
The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.
Want to see the full answer?
Check out a sample textbook solutionChapter 14 Solutions
BIOCHEMISTRY-ACHIEVE (1 TERM)
- SIGNALS AND TARGETS. Listed below are sample polypeptides/proteins with their signal molecule/peptide. Answer the questions that follow. If you are asked to give the amino acid sequence, provide the sequence using the three-letter names of the amino acids (eg. ser-ala-met). Protease with mannose-6-phosphate Where is the receptor for this protein located? Where is the final destination of this polypeptide? What happens to the receptor after protein transport?arrow_forwardLigand binding and response. The following question involves the ligand binding to a receptor and the receptor's response to that ligand. What ligand concentration would be required for a full agonist with a KD of 8 nM to achieve a response of 0.75?arrow_forwardSIGNALS AND TARGETS. Listed below are sample polypeptides/proteins with their signal molecule/peptide. Answer the questions that follow. If you are asked to give the amino acid sequence, provide the sequence using the three-letter names of the amino acids (eg. ser-ala-met). Polymerase with H2N-...GMMTVPPKKKRVGMMTV...-COOH Provide the amino acid sequence of the signal peptide Where will this polypeptide be transported? What is the receptor of the signal sequence? What is the transport complex for this protein?arrow_forward
- Signal transduction pathway. Sketch a G protein in the active and inactive stages, and label its parts.arrow_forwardPlease help me with this question. More than one answer may be correct. THe graph relating to the information is included below. The figure shows the number of cells that have clusters of IRE1 molecules after those cells are treated with various levels of thapsigargin (Tg), a chemical that can induce ER stress. IRE1 can form these clusters when ER stress is induced and this clustering can cause activation of RNAse activity in IRE1. In this experiment, normal IRE1 was used (IRE1α) that can bind to Sec61, along with a modified version of IRE1 that binds to Sec61 more weakly than normal IRE1 (wIRE1α), and another modified version that binds to Sec61 more strongly than normal IRE1 (sIRE1α). From this figure you can conclude that: Question 18 options: IRE1 binding to Sec61 promotes the formation of IRE1 clumps IRE1 binding to Sec61 prevents the formation of IRE1 clumps co-translational translocation is a key process the golgi aparatus is heavily involved in the unfolded protein response The…arrow_forwardPlease help me with this question. More than one answer may be correct. THe graph relating to the information is included below. The above figures show the rate of actin polymerization in the presence of various concentration of profilin (Pfn). In the top figure, flourescence intensity is a measure of total actin that has been polymerized, and this is plotted versus time in seconds. The shade of blue of the lines in the top figure correspond to the shaded blue bars representing various concentrations of profilin in the lower figure. The lower graph shows the initial rates of polymerization of actin plotted again concentration. Which of the following is true: Question 21 options: profilin in a molecular motor profilin is a promoter of actin polymerization profilin replaced G-actin in an F-actin strand and breaks the filament profilin is an inhibitor of actin polymerization profilin binds to G-actin, preventing it from polymerizingarrow_forward
- Crohn’s disease. Omega-3 fatty acids have been tested asa means to prevent relapse of Crohn’s disease. Two large,randomized, placebo-controlled studies have shown nosuch benefit from omega-3 fatty acids. Suppose you areasked to design an experiment to further study this claim.Imagine that you have collected data on Crohn’s relapsesin subjects who have used these omega-3 fatty acids and similar subjects who have not used them and that you canmeasure incidences of relapse for these subjects. Statethe null and alternative hypotheses you would use in yourstudy.arrow_forwardSIGNALS AND TARGETS. Listed below are sample polypeptides/proteins with their signal molecule/peptide. Answer the questions that follow. If you are asked to give the amino acid sequence, provide the sequence using the three-letter names of the amino acids (eg. ser-ala-met). Catalase with H2N-...KERINGKERIANGEKSAMSKL-COOH Provide the amino acid sequence of the signal peptide Where will this polypeptide be transported? (specify the compartment) What is the name of the specific receptor of this polypeptide? The receptor may also have what alternative function?arrow_forwardHelp me fast with detail explanation. Definitely I will give Upvote.arrow_forward
- = 20 nM. The rate of receptor-ligand complex formation with an A receptor-ligand complex has a dissociation constant of Ka added ligand concentration of 10 µM is 5 × 10³ s¯¹. What is the value of the reverse rate constant, k_₁ ? k_₁ = 8-1arrow_forwardBiotransformation. Explain the process of enzyme induction. What are the benefits or down-falls of this process.arrow_forwardTPA protein function. Use at least two sentences to describe the function of the TPA protein.arrow_forward
- BiochemistryBiochemistryISBN:9781319114671Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.Publisher:W. H. FreemanLehninger Principles of BiochemistryBiochemistryISBN:9781464126116Author:David L. Nelson, Michael M. CoxPublisher:W. H. FreemanFundamentals of Biochemistry: Life at the Molecul...BiochemistryISBN:9781118918401Author:Donald Voet, Judith G. Voet, Charlotte W. PrattPublisher:WILEY
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningFundamentals of General, Organic, and Biological ...BiochemistryISBN:9780134015187Author:John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. PetersonPublisher:PEARSON