Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 14, Problem 4TY
Summary Introduction
Introduction: Bacterial DNA (Deoxyribose
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If you want to express a eukaryotic gene in a bacterial cell, you ned to (select all that apply):
a.) add a bacterial promoter in front of the gene
b.) remove introns from the gene
c.) add a polyA tail sequence
d.) add eukaryotic tRNAs
The gad operon is controlled by a number of transcription factors that regulate a promoter of approximately 450bp. Which would NOT explain why bacterial promoters are shorter than the typical eukaryotic promoter?
a. Bacteria do not have nucleosomes
b. Bacteria do not have mediator complex
c. Each operon is regulated by only one signal
d. All of the above are true
In gene silencing, the “dicer” enzymea. assembles siRNAs into a RISC complex.b. unwinds the RISC complex.c. binds siRNA to mRNA sequences complementary to it.d. slices up double-stranded RNA molecules
Chapter 14 Solutions
Biology
Ch. 14.1 - Prob. 1CCCh. 14.2 - Which genes are under the control of the lac...Ch. 14.2 - With regard to regulatory proteins and small...Ch. 14.2 - What were the key observations made by Jacob,...Ch. 14.2 - CoreSKILL What was the eventual hypothesis...Ch. 14.2 - Prob. 3EQCh. 14.2 - Core Skill: Connections Look back at Fig 9.12....Ch. 14.2 - What are the advantages of having both an...Ch. 14.2 - Prob. 2CSCh. 14.3 - Prob. 1CC
Ch. 14.4 - What are the two opposing effects that histone...Ch. 14.4 - Prob. 1CSCh. 14.5 - Prob. 1CCCh. 14.5 - Prob. 2CCCh. 14 - Prob. 1TYCh. 14 - Prob. 2TYCh. 14 - Transcription factors that bind to DNA and...Ch. 14 - Prob. 4TYCh. 14 - For the lac operon, what would be the expected...Ch. 14 - Prob. 6TYCh. 14 - The trp operon is considered _____ blank operon...Ch. 14 - Prob. 8TYCh. 14 - Prob. 9TYCh. 14 - _____ blank refers to the process that allows a...Ch. 14 - Prob. 1CQCh. 14 - Transcriptional regulation often involves a...Ch. 14 - Prob. 3CQCh. 14 - Discuss the advantages and disadvantages of...Ch. 14 - Discuss the advantages and disadvantages of...
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- In the trp operon, if a mutation occurred in the gene for the repressor so that it could bind to the operator even in the absence of tryptophan____________. a) the inducer cannot bind trpO, so operon gene transcription occurs b) the active repressor cannot bind trpO, so operon gene transcription is attenuated c)the active repressor binds tpO, so operon gene transcription is always repressed d) the repressor binds the corepressor, and operon gene transcription occurs.arrow_forwarda. What is the function of operons in bacterial gene regulation? b. Describe how a bacterial operon can be regulated by repressible proteins (such as the tryptophan operon). Include in your description both the “on” and “off” states of the operon. Key elements of your diagram should include: Co-repressor, Genes, mRNA, Operator, Operon, Promoter, Repressor, RNA polymerase.arrow_forwardA mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcriptionarrow_forward
- Which of the following is characteristic of genes and gene regulation in both bacteria and eukaryotes? (a) promoters (b) non-coding DNA within coding sequences (c) enhancers (d) operons (e) DNA located in a nucleusarrow_forwardWhat is the difference between the core promoter and the regulatory promoter? a. Only the core promoter has consensus sequences. b. The regulatory promoter is farther upstream of the gene. c. General transcription factors bind to the core promoter; transcriptional activator proteins bind to the regulatory promoter. d. Both b and c.arrow_forwardf you made a change in the promoter sequence in the DNA that inactivates the promoter, what would happen at the RNA level? A-Nothing, because the RNA would be made as usual B-Transcription factors would be unable to bind and the RNA polymerase would not be recruited to the DNA, so no RNA would be made. C-The mutation of the DNA would be carried through to the RNA sequence. D-The DNA helicase would not be able to recognize and bind the DNA, so the RNA would not be made. EXPLAIN WHY THE ANSWER YOU CHOOSE IS CORRECTarrow_forward
- One strategy for producing a protein in the milk of a cow is toplace the coding sequence of the gene of interest next to a__________ and then inject the gene into a _________.a. lac operon promoter, cow oocyteb. β-lactoglobulin promoter, cow oocytec. lac operon promoter, cow mammary celld. β-lactoglobulin promoter, cow mammary cellarrow_forwardWhich of the following is true about operons? A. Contains a cluster of genes transcribed as multiple mRNAs. B. Contains a cluster of genes transcribed as a single mRNA. C. They are commonly found in eukaryotes. D. Can only be regulated by gene activator proteins.arrow_forwardPart A: During cloning, the DNA is cut with a restriction enzyme giving it what? A. more introns B. sticky ends C. a polyA tail D. a binding site for a transcription factor Part B: If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use? A. PCR B. epigenetics C. hybridization D. gel electrophoresis Part C: If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible? A. transcription factor B. rRNA C. ribosome D. RNA polymerase Part D: If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what? A. PCR B. gene therapy C. stem cell work D. DNA fingerprintingarrow_forward
- In the lac operon, the lac genes are transcribed in the presence of lactose because a RNA polymerase binds to the operator b The repressor can not bind the promoter c Production of the repressor protein is inducible d The inducer binds to the repressor e The inducer binds to the operatorarrow_forwardd. give an example of an operon that is under negative control e. repressor f. give an example of a repressor molecule g. activator h. give an example of an activatorarrow_forwardWhich of the following statements best describes the promoter of a protein-coding gene? A. The promoter is a protein that recruits RNA polymerase. B. The promoter is part of the RNA molecule itself. C. The promoter is a site found on RNA polymerase. D. The promoter contains the AUG start codon. E. The promoter is a nontranscribed region of a gene.arrow_forward
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