Biology
Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 14, Problem 5TY

 For the lac operon, what would be the expected effects of a mutation in the operator site that prevented the binding of the repressor protein?

  1. a. The operon would always be turned on.
  2. b. The operon would always be turned off.
  3. c. The operon would always be turned on, except when glucose is present.
  4. d. The operon would be turned on only in the presence of lactose.
  5. e. The operon would be turned on only in the presence of lactose and the absence of glucose.
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Gene expression in bacteria is controlled by the operon model.  a) Why is the operon model important? b) Why is the trp operon known as a repressible operon?
If β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon?   a)  There is a mutation in the repressor protein   b)  There is a mutation in the catabolite activator protein   c)  There is a mutation in the promoter region   d)  There is a mutation in the luxI protein
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan.  Using your knowledge of operons and their regulation   a.Which operons would be functional under these conditions? b.What repressors would be made? (NOTE: name the repressors using their gene names) c.Which repressor(s) would be made in the inactive form? d.Which repressor(s) would be made in the active form? e.Which repressor(s) under these conditions can bind the operator sequence? f.Which repressor(s)under these conditions cannot bind the operator sequence?
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