Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Textbook Question
Chapter 14, Problem 5TY
For the lac operon, what would be the expected effects of a mutation in the operator site that prevented the binding of the repressor protein?
- a. The operon would always be turned on.
- b. The operon would always be turned off.
- c. The operon would always be turned on, except when glucose is present.
- d. The operon would be turned on only in the presence of lactose.
- e. The operon would be turned on only in the presence of lactose and the absence of glucose.
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Gene expression in bacteria is controlled by the operon model.
a) Why is the operon model important?
b) Why is the trp operon known as a repressible operon?
If β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon?
a)
There is a mutation in the repressor protein
b)
There is a mutation in the catabolite activator protein
c)
There is a mutation in the promoter region
d)
There is a mutation in the luxI protein
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation
a.Which operons would be functional under these conditions?
b.What repressors would be made? (NOTE: name the repressors using their gene names)
c.Which repressor(s) would be made in the inactive form?
d.Which repressor(s) would be made in the active form?
e.Which repressor(s) under these conditions can bind the operator sequence?
f.Which repressor(s)under these conditions cannot bind the operator sequence?
Chapter 14 Solutions
Biology
Ch. 14.1 - Prob. 1CCCh. 14.2 - Which genes are under the control of the lac...Ch. 14.2 - With regard to regulatory proteins and small...Ch. 14.2 - What were the key observations made by Jacob,...Ch. 14.2 - CoreSKILL What was the eventual hypothesis...Ch. 14.2 - Prob. 3EQCh. 14.2 - Core Skill: Connections Look back at Fig 9.12....Ch. 14.2 - What are the advantages of having both an...Ch. 14.2 - Prob. 2CSCh. 14.3 - Prob. 1CC
Ch. 14.4 - What are the two opposing effects that histone...Ch. 14.4 - Prob. 1CSCh. 14.5 - Prob. 1CCCh. 14.5 - Prob. 2CCCh. 14 - Prob. 1TYCh. 14 - Prob. 2TYCh. 14 - Transcription factors that bind to DNA and...Ch. 14 - Prob. 4TYCh. 14 - For the lac operon, what would be the expected...Ch. 14 - Prob. 6TYCh. 14 - The trp operon is considered _____ blank operon...Ch. 14 - Prob. 8TYCh. 14 - Prob. 9TYCh. 14 - _____ blank refers to the process that allows a...Ch. 14 - Prob. 1CQCh. 14 - Transcriptional regulation often involves a...Ch. 14 - Prob. 3CQCh. 14 - Discuss the advantages and disadvantages of...Ch. 14 - Discuss the advantages and disadvantages of...
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- In the trp operon, if a mutation occurred in the gene for the repressor so that it could bind to the operator even in the absence of tryptophan____________. a) the inducer cannot bind trpO, so operon gene transcription occurs b) the active repressor cannot bind trpO, so operon gene transcription is attenuated c)the active repressor binds tpO, so operon gene transcription is always repressed d) the repressor binds the corepressor, and operon gene transcription occurs.arrow_forwardWhat would occur if the repressor of an inducible operon weremutated so it could not bind the operator?(A) irreversible binding of the repressor to the promoter(B) reduced transcription of the operon’s genes(C) buildup of a substrate for the pathway controlled by theoperon(D) continuous transcription of the operon’s genesarrow_forwardWhat is the role of a substrate that controls a repressible operon? a. increase the production of inactive repressor proteins b. bind to the repressor protein and activate it c. bind to the repressor protein and deactivate it d. bind to the promoter region and decrease the affinity of RNA polymerase for the promoterarrow_forward
- How does tryptophan regulates its own production? a. When tryptophan levels are high, it binds to the trp repressor, which changes its shape and binds to the operator sequence in the trp operon. b. When tryptophan levels are high, it binds to the trp repressor, which changes its shape and binds to the promoter sequence in the trp operon. c. When tryptophan levels are high, it binds to the trp repressor, which changes its shape and falls off from the operator sequence in the trp operon. d. When tryptophan levels are high, it binds to the trp repressor, which changes its shape and falls off from the promoter sequence in the trp operon.arrow_forwardA mutation in the operator region of the trp operon can prevent the trp repressor from binding to this operator. When these mutant cells are placed in a solution containing chicken broth, which of the following happens? a. Tryptophan would bind to the repressor. b. The Repressor would bind to the operator irrespective of the presence of amino acids in the environment. c. The transcription of the trip operon would be inhibited. d. The transcription of the repressor protein is inhibitedarrow_forwardHow does the lac operon regulate lactose digestion in bacteria?a. The repressor protein becomes a lactose-digesting enzyme onlywhen lactose is present.b. The repressor protein binds to the lac operon when lactose ispresent, blocking transcription.c. When lactose is present, it binds to the operator region of the lacoperon, activating transcription of the repressor protein gene.d. The repressor protein falls off the lac operon when lactose ispresent, and lactose-digesting genes are expressed.arrow_forward
- If you add a co-eepressor to a repressor protein and that allows it to bind the operator is this a inducible, constitutive or repressible operon?arrow_forwardWhich of these BEST DESCRIBE tryptophan in the Trp Operon? A. Acts as a corepressor B. Acts as a coactivator C. Acts as an inducer D. Acts as an enhancerarrow_forwardIf you made a mutation in the operator region of the lac promoter such that LacI can no longer bind to it, what would be the effect on lac operon expression?arrow_forward
- A researcher engineers a lac operon on a plasmid but inactivates all parts of the lac operator (lacO) and the lac promoter, replacing them with the binding site for the LexA repressor (which acts in SOS response) and a promoter regulated by LexA. The plasmid is introduced into E. coli cells that have a lac operon with an inactive lacZ gene. Under what conditions will these cells produce beta-galactosidase?arrow_forwardWhich will be bound to the Lac Operon in an environment with lactose and glucose? A) both repressor and CAP B) repressor only C) CAP only D) neither repressor nor CAParrow_forwardWhat is an operon ? a. A series of genes controlled by the same operator b. A series of genes on the same mRNA molecule c. A series of genes that all have the same function d. A series of genes all regulated by the same repressorarrow_forward
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