Concept explainers
DNA Base Composition. Based on your understanding of the rules of complementary base pairing, answer the following questions:
(a) You analyze a DNA sample and find that its base composition is 30% A, 20% T, 30% G, and 20% C. What can you conclude about the structure of this DNA?
(b) For a double-stranded DNA molecule in which 40% of the bases are either G or C, what can you conclude about its content of the base A?
(c) For a double-stranded DNA molecule in which 40% of the bases are either G or T, what can you conclude about its content of the base A?
(d) For a double-stranded DNA molecule in which 15% of the bases are A, what can you conclude about its content of the base C? Would you expect the Tm of this DNA to be above or below 90°C? (Hint: Refer to Figure 16-12.)
Want to see the full answer?
Check out a sample textbook solutionChapter 16 Solutions
WORLD OF CELL+MASTERING ACCESS >CUSTOM
- Number of Okazaki Fragments in E. coli and Human DNA Replication Approximately how many Okazaki fragments are synthesized in the course of replicating an E. coli chromosome? How many in replicating an “average� human chromosome?arrow_forwardReplication:- what other enzymes are involved in the initiation phase?- explain the role of primers in this phase- how is the building of the leading strand different from that of the lagging strand?arrow_forwardComposition as a mole fraction of one of a double-stranded DNA strand T = 0.22 and C = 0.30. In the light of this information, the following values are Calculate as a fraction. If the given information is used to calculate the desired value, If it is not sufficient, indicate the result as X.arrow_forward
- DNA What do A and G have in common that is notably different from T and C?arrow_forwardNot just generic "degradation" or even shorter DNA fragments, what specific structure change in double-stranded DNA does the hyperchromicity effect show?arrow_forwardThinking about the complexity of biochemical systems as they relate to the human body and the specificity of DNA, why can we not describe the “average” behavior of a DNA molecule?arrow_forward
- recall that DNA has bases A, C, G, and T. Suppose we invented a DNA2 genetic material that has bases A, A1, C, C1, G, G1, T and T1. then how many symbols can be represented by two characters from DNA2?arrow_forwardVertebrate proteins? What is meant by the term polypeptide backbone?arrow_forwardCompare and contrast the structure of DNA and RNA. Be sure to describe each of the three components of a nucleotide for both DNA and RNA along with the types of bonds formed between the components. In addition, explain: how the nucleotides link together to form each molecule, why the prime ends are labeled 5’ and 3’, what antiparallel is, what phospodiester linkages are and what complementary base pairing is.arrow_forward
- Question- Determine the exact molecular weight of the following oligonucleotide and calculate the average molecular weight of an equivalently long oligonucleotide. What is the percent error between the two? Sequence (5’-3’) : GCT TTA GCG GTC AAG TGC TGT GCC ATT T M.W. of a nucleotide in ssDNA = 303.7 + 79.0 M.W. of base pair in dsDNA = 607.4 + 158arrow_forwardTick the correct statements: Remember: Tautomers are structural isomers that differ from each other based on the position of the proton(s) and their double bonds. ( ) The nitrogenous bases of nucleic acids, which contain heterocyclic and analogous nuclei, can adopt different tautomeric forms involving multiple H+ that are exchangeable depending on the medium. In DNA, spontaneous formation of smaller tautomers appears to contribute to mutagenic errors during DNA replication, while in RNA, they seem to be related to increased structural and functional diversity of enzymes and RNA aptamers (research this and confirm if it is false or real) ( ) in relation to the figure, anomer 1 has beta stereochemistry with respect to the C anomeric of the pentose, and is making an N-glycosidic bond ( ) in relation to the figure, anomer 1 has alpha stereochemistry with respect to the C anomeric of the pentose, and is making an N-glycosidic bond ( ) In general, in naturally occurring nucleosides,…arrow_forwardDNA What do thymine (T) and cytosine (C) have in common, that makes them different from Adenine (A), and Guanine (G)?arrow_forward
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage LearningBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax