WORLD OF CELL+MASTERING ACCESS >CUSTOM
WORLD OF CELL+MASTERING ACCESS >CUSTOM
9th Edition
ISBN: 9781323445044
Author: Hardin
Publisher: PEARSON C
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Chapter 16, Problem 16.4PS

QUANTITATIVE DNA Melting. Figure 16-36 shows the melting curves for two DNA samples that were thermally denatured under the same conditions.

Chapter 16, Problem 16.4PS, QUANTITATIVE DNA Melting. Figure 16-36 shows the melting curves for two DNA samples that were

Figure 16-36 Thermal Denaturation of Two DNA Samples. See Problem 16-4.

  1. (a) What conclusion can you draw concerning the base compositions of the two samples? Explain.
  2. (b) How might you explain the steeper slope of the melting curve for sample A?
  3. (c) Formamide and urea are agents known to form hydrogen bonds with pyrimidines and purines. What effect, if any, would the inclusion of a small amount of formamide or urea in the incubation mixture have on the melting curves?
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