(a)
Interpretation:
The difference between the phosphofructokinase (PFK) activity in P. Furiosus and other animals should be determined.
Concept introduction:
Phosphofructokinase is an allosteric enzyme which converts fructose-1-phopshate to fructose-1,6-bisphosphate. It acts as a regulatory enzyme in the glycolysis process. High ATP concentration inside the cell inhibits the activity of PFK.
(b)
Interpretation:
Effect of ATP and AMP on ADP should be determined.
Concept introduction:
Phosphofructokinase is an allosteric enzyme which converts fructose-1-phopshate to fructose-1,6-bisphosphate. It acts as a regulatory enzyme in the glycolysis process. High ATP concentration inside the cell inhibits the activity of PFK.
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Chapter 16 Solutions
BIOCHEMISTRY
- Shown below is a substrate for a Trypsin. Draw the mechanism for this serine protease using the artificial substrate. Be sure to draw the catalytic triad, and show the role of the oxyanion hole. Draw the complete structure of every intermediate and product and PUSH ARROWS!!!!! Do not abbreviate structures using R and R' H₂N _N_CH. сно CH₂ CH₂ CH₂ NH d=19H₂ NH₂ O CH- H₂C HN O CHarrow_forwardKindly refer to the photo attached. thank youarrow_forwardH. OH co co2 но H co, 1-isopropylmalate 2-isopropylmalate Biosynthesis of leucine involves conversion of 1-isopropyimalate to 2-isopropylmalate (see above). This proceeds in four steps under basic enzymic catalysis via an isolable compound produced in step 2. Write a detailed mechanism for this conversion. Then, draw the intermediate compound) produced in step 2. • You do not have to consider stereochemistry. • Draw uninvolved carboxyl groups in the anionic state, and enolates as carbanions. When needed, abbreviate CoenzymeA-S- as CH3S- In your drawing. aalearrow_forward
- Need help Sesamin is one of the major lignans found in sesame oil. Sesamin-metabolic pathways remain uncharacterized at both the enzyme and gene levels. However, one microorganism showed significant sesamin-degrading activity and was identified as Sinomonas sp. no. 22. A sesamin- metabolizing enzyme named SesA was purified from this strain and characterized. Explain how sesamin methylene transferase was identified and characterized and explain the mechanism. Based on your knowledge, what other assays could be run to characterize this enzyme?arrow_forwardHand written solutions are strictly prohibited.arrow_forwardplease explain. I will rate your response based on the accuracy of your answers. Two right answers get a thumbs up rating! Thank you.arrow_forward
- Need help, please.arrow_forwardUsing the ActiveModel for aldose reductase, describe the structure of the TIM barrel motif and the structure and location of the active site.arrow_forwardUsing the ActiveModel for enoyl-CoA dehydratase, give an example of a case in which conserved residues in slightly different positions can change the catalytic rate of reaction.arrow_forward
- Extending the Mechanism of Methylmalonyl-CoA Mutase to Similar Reactions Based on the mechanism for the methylmalonyl-CoA mutase (see problem 14), write reasonable mechanisms for the following reactions shown.arrow_forwardHelp me answer 5, 6 and 7 subunits. Thank you!arrow_forwardPlease answer all parts in detail. Give explainations. Thanks.arrow_forward
- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning