Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 16.1, Problem 2R
Summary Introduction
To review:
The comparison of molecular conditions that occur at the lac operon promoter in the absence as well as the presence of lactose.
Introduction:
The operon is generally a cluster of genes containing a single promoter. The operon found in Escherichia coli which encodes three lactose
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Explain why catabolite repression is used in regulating the lac operon and describe how it fine-tunes b-galactosidase synthesis.
Explain how the data shown indicate that two operator sites are necessary for repression of the lac operon. What would the results have been if all three operator sites were required for the binding of lac repressor?
Identify the different functions of the genes coded in the lac operon region.
Chapter 16 Solutions
Life: The Science of Biology
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- Compare the arrangement of cis-acting sites in the control regions of the lac operon and phage λarrow_forwardDescribe the binding of RNA polymerase, repressors, andactivators to the lac operon when both lactose and glucose are scarce. What is the effect of these scarcities ontranscription of the lac operon?arrow_forwardExplain how regulatory regions of the lac operon canbe used in the production of pharmaceutical proteinsarrow_forward
- Describe the probable genetic defect that abolishes the sensitivity of the lac operon to the absence of glucose when other metabolic operons continue to be sensitive to the absence of glucose.arrow_forwardExplain how the lac operon is both positively and negativelycontrolled.arrow_forwardIdentify the elements of the LAC operonarrow_forward
- Diagram the lactose operon in E. coli with the properorder of the elements lacI, lacO, lacY, and lacZ, and explainhow expression is controlled in the presence and in theabsence of lactose.arrow_forwardDescribe the organization of the lac operon.arrow_forwardDescribe the regulation of the lac operon when lactose is present and glucose is absentarrow_forward
- Under what conditions would you expect to see high levels of expression of the structural genes in the lac operon? Group of answer choices Lactose is absent and glucose is absent Lactose is absent and glucose is present Lactose is present and glucose is present None of these Lactose is present and glucose is absentarrow_forwardExplain why (a) inactivation of the O2 or O3 sequence of the lac operon causes only a twofold loss in repression, and (b) inactivation of both O2 and O3 reduces repression ∼70-fold.arrow_forwardExplain why inactivation of the O1 sequence of the lac operator almost completely abolishes repression of the lac operon.arrow_forward
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