Life: The Science of Biology
Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 16.1, Problem 4R
Summary Introduction

To review:

The efficiency of the promoter for the repressor protein and whether the synthesis of the repressor is constitutive or inducible.

Introduction:

The regulatory proteins bind to the DNA (deoxyribonucleic acid) and play a key role in determining which genes are to be activated. These are of two types, namely, the repressor and activator proteins. The proteins, whose synthesis is regulated by inducers are known as inducible proteins, while the proteins that are synthesized at a constant rate all the time are known as constitutive proteins.

In the given case, a regulatory gene codes for the repressor protein, which is synthesized in small quantities at a constant rate. This protein acts on the lac operon of Escherichia coli (E. coli). A promoter is a DNA region, which initiates the process of transcription of a certain gene by binding with the ribonucleic acid (RNA) polymerase.

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In the lac operon (below), how will expression of the genes lacZ and lacy be effect by a mutation in the repressor protein (encoded by lach) that prevents it from binding the operator sequence (lacO) in the absence of lactose? Explain the answer
If CAP is bound to the Promoter of the Lac Operon and the repressor protein is not bound to the operator, which of the following is true: a.Glucose Levels are low & Lactose is absent b.Glucose is absent & Lactose is present c.cAMP is low & Lactose is Absent d.Glucose and Lactose are Present e.cAMP is low & Lactose is present
If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not alter the conformation of the repressor protein, how would the expression of the lac operon be affected?
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