Concept explainers
To review:
The efficiency of the promoter for the repressor protein and whether the synthesis of the repressor is constitutive or inducible.
Introduction:
The regulatory proteins bind to the DNA (deoxyribonucleic acid) and play a key role in determining which genes are to be activated. These are of two types, namely, the repressor and activator proteins. The proteins, whose synthesis is regulated by inducers are known as inducible proteins, while the proteins that are synthesized at a constant rate all the time are known as constitutive proteins.
In the given case, a regulatory gene codes for the repressor protein, which is synthesized in small quantities at a constant rate. This protein acts on the lac operon of Escherichia coli (E. coli). A promoter is a DNA region, which initiates the process of transcription of a certain gene by binding with the ribonucleic acid (RNA) polymerase.
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Life: The Science of Biology
- Describe how the components of the trp operon system are affected by a mutation in trpC? Why is it that once enviornmental tryptophan is inserted into the cell, only the trp repressor is activated (turned on)?arrow_forwardMutations may have an effect on the expression of the lac operon and the trp operon. Would the following mutations have a cis- or transeffect on the expression of the protein-encoding genes in the operon? A. A mutation in the operator site that prevents lac repressor from binding to it B. A mutation in the lacI gene that prevents lac repressor from binding to DNA C. A mutation in the trpL gene that prevents attenuationarrow_forwardAssuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in_____ Constitutive expression of the lac operon genes ito sagot ko Lack of expression or reduced expression of the lac operon genes under all circumstances Expression of the genes only when lactose is present Expression of the genes only when lactose is absentarrow_forward
- What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)arrow_forwardThe lac operon produces enzymes that allow the bacteria E. coli to metabolize lactose, it is in an inducable operon. When lactose is present, will the repressor protein attach or leave the operon? How would this effect the production of lactose producing enzymes?arrow_forwardThe lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) IS O+ Z+ IS OC Z+arrow_forward
- The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) I+ OC Z+ I+ O+ Z+ (Lactose present) I- O+ Z+arrow_forwardIf the above gene is one of the three structural genes of the lac operon that codes for the protein/ enzyme responsible for breaking lactose into two molecules of simple sugars, what triggers the activation of this gene? a. Absence of Inhibitory protein b. Presence of lactose c. Absence of lactose d. Presence of Inhibitory protein e. Absence of Regulatory proteinarrow_forwardIn the lac operon system, lactose is considered the ___________, and when the gene lacI is mutated such that the protein it produces cannot bind to lactose (IS mutation), the lac operon is ________. Group of answer choices repressor; never expressed activator; constitutively expressed inducer; never expressed inducer; constitutively expressed structural gene; never expressedarrow_forward
- In the lac operon, the lacZ gene codes for an enzyme called ____________, which catalyze the breakdown of the sugar __________ into monosaccharides. This enzyme can also hydrolyze the sugar analog ____________, which produces blue pigments following its breakdown. Since the lac operon is not always on the bacterial cells, we add the chemical ________ which prevents the lac depressor from binding to the operators, leading to constitutive expression of the operon.arrow_forwardA mutation in the operator region of the trp operon can prevent the trp repressor from binding to this operator. When these mutant cells are placed in a solution containing chicken broth, which of the following happens? a. Tryptophan would bind to the repressor. b. The Repressor would bind to the operator irrespective of the presence of amino acids in the environment. c. The transcription of the trip operon would be inhibited. d. The transcription of the repressor protein is inhibitedarrow_forwardIf β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon? a) There is a mutation in the repressor protein b) There is a mutation in the catabolite activator protein c) There is a mutation in the promoter region d) There is a mutation in the luxI proteinarrow_forward
- Biology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage Learning