BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 17, Problem 2DA
Summary Introduction
To determine: The number of spots that would be observed when human cells in the G2 phase of the cell cycle are subjected to the FISH analysis using the probe recognizing telomeric DNA.
Introduction: Fluorescent in situ hybridization (FISH) is used to identify gross chromosomal rearrangements. It is a molecular cytogenic technique in which fluorescent probes are used to detect DNA sequences.
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During prophase of mitosis
Select one:
A.the spindle breaks down.
B.nucleoli and the nuclear envelope begin to break down.
C.chromatids divide.
The interval before the onset of DNA replication is
Select one:
A.the G1 stage.
B.the S phase.
C. the G2 stage.
Metaphase- Mitosis
a) Chromosomes line up at the equator of the cell.
b) One spindle fiber of the very conspicuous spindle system attaches to the centromere of each chromosome.
Each metaphase chromatid is about 700 nm in diameter. How many times wider is that than a DNA double helix? Show your work.
Chapter 17 Solutions
BIOLOGY
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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- Biologists have long been interested in the effects of radiation on cells. In one experiment, researchers examined the effect of radium on mitosis of chick embryo cells growing in culture. A population of experimental cells was examined under the microscope for the number of cells in telophase (as a measure of mitosis occurring) before, during, and after exposure to radium. The results are shown in the Figure. What is the effect of radium exposure on mitosis? Source: R. G. Canti and M. Donaldson. 1926. The effect of radium on mitosis in vitro. Proceedings of the Royal Society of London, Series B, Containing Papers of a Biological Character 100:413419.arrow_forwardIn the tracking chromosomal DNA movement through mitosis experiment, how many chromosomes did each of your daughter cells contain? Why is it important for each daughter cell to contain information identical to the parent cell? How often do human skin cells divide? Why might that be? Compare this rate to how frequently human neurons divide. What do you notice?arrow_forwardAmount of DNA per nucleus over the cell division cycle. At which point in the accompanying figure does MPF reach its highest activity during the cell division cycle? a) I b) II c) III d) IVarrow_forward
- Compare and contrast the structure of DNA and RNA. During Prophase of Mitosis, chromosomes begin to condense and are fully condensed by the time the cell is in Metaphase of Mitosis. Explain how the chromatin organizes itself from the beginning of prophase to metaphase of mitosis.arrow_forwardA scientist adds colchicine, a microtubule polymerization inhibitor, to a cell entering mitosis. At metaphase, the chromosomes of the cell were arranged as shown below. Explain why this arrangement is consistent with the application of colchicine. Predict how colchicine would affect the cell as it attempts mitosis.arrow_forwardOncologists (cancer doctors) frequently measure the mitotic index of tumor cells when they are removed via surgery or biopsy. What do you think is the value of finding out the mitotic index of these cells?arrow_forward
- Observation in whitefish blastula mitosis (experiment), like it is in anaphase, prophase or other stages that can be seen through microscope. Explain it. Thanksarrow_forwardMITOSIS: Why is each cell stage a prerequisite to the next?arrow_forwardMETAPHASE OF ONION ROOT CELL (PLANT CELL) describe the appearance of DNA, spindle fibers and location of the chromosomes please describe the appearance and location, do not state the process do not copy from google pleasearrow_forward
- You are interested in testing the effect of a drug on human cells. These cells have been sampled and evenly split into two separate cell cultures. You trigger cell division using a growth factor in both cell cultures. This synchronises the start of mitosis so you can easily detect the effect of the drug on the progress through the mitotic phases. Immediately after mitosis begins, you expose one cell culture to the drug (treatment + drug) and compare their progress through the mitotic phases under the microscope to the cells that are not exposed to the drug (control - drug). Here are your observations: Cells from both cell cultures lack their nuclear membranes. Individual chromosomes are clearly visible in both cell cultures Cells from the control culture (- drug) have their chromosomes aligned (see figure below) Cells from the treatment (+ drug) have their chromosomes dispersed in the cell (see figure below). What effect does the drug have on the chromosomes? Indicate two…arrow_forwardIn the mitosis experiment, in what stage were most of the onion root tip cells in? Based on what you know about cell cycle division, what does this imply about the life span of a cell? Were there any stages of the cell cycle that you did not observe? How can you explain this using evidence from the cell cycle?arrow_forwardScientists often extract DNA from the nucleus of cells for analysis or use. This process breaks the cell membrane, spins the solution to remove the large particles through high g-force, adds alcohol to make the DNA less soluble aqueous solution, then spins the solution again to pull the DNA out of solution. Consider a cell in metaphase compared to a cell in rest (not in the cell cycle leading to cell division). What properties of a metaphase cell might let you extract more DNA compared to the resting cell? Are there any that might make the extraction more difficult?arrow_forward
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