BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 17, Problem 6U
The Ti plasmid of Agrobacterium usually induces tumors called galls on infected plants. When Agrobacterium is used to make transgenic plants, why does no gall form?
a. The introduced transgene blocks the action of the gall-inducing genes.
b. The gall-inducing genes are removed and replaced with the transgene.
c. The Ti plasmid is removed from Agrobacterium used to make transgenic plants.
d. The Ti plasmid is mutated by PCR so that it cannot replicate in infected plants.
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Which technique allows a researcher to change a single specific nucleotide in a gene sequence in vitro?
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The CRISPR-Cas9 system
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PCR-based random mutagenesis
c.
PCR-based site-specific mutagenesis
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RT-PCR
Which method is used to obtain mutants that grow under conditions that the wild type parent cannot grow?
a)indirect selction
b) direct selection
c) screening for possible mutagen ( carcinogens)
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If you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene?
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insert the known gene into a vector and use the vector to insert the known gene into the other organism
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treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis
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create a hybrid of the two organisms by breeding them and check for mutations
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create labeled DNA probes from the known gene and use them to search the genome of the other organism
Chapter 17 Solutions
BIOLOGY
Ch. 17.1 - Prob. 1LOCh. 17.1 - Prob. 2LOCh. 17.1 - Describe the construction and uses of recombinant...Ch. 17.2 - Relate the process of DNA replication to PCR.Ch. 17.2 - Compare and contrast PCR, RT-PCR, and quantitative...Ch. 17.3 - Prob. 1LOCh. 17.3 - Prob. 2LOCh. 17.3 - Describe the pros and cons of RNA interference and...Ch. 17.4 - Explain how the universal nature of the genetic...Ch. 17.4 - Compare and contrast knockout, knockin, and...
Ch. 17.4 - Prob. 3LOCh. 17.5 - Describe the benefits of biofuel production from...Ch. 17.5 - Prob. 2LOCh. 17.5 - Prob. 3LOCh. 17.6 - Prob. 1LOCh. 17.6 - Compare and contrast FISH and gene chip...Ch. 17.6 - Describe how immunoassays can be used to diagnose...Ch. 17.7 - Describe the benefits of creating transgenic...Ch. 17.7 - Prob. 2LOCh. 17.7 - Evaluate issues on each side of the transgenic...Ch. 17 - Prob. 1DACh. 17 - Prob. 2DACh. 17 - Prob. 1IQCh. 17 - Prob. 2IQCh. 17 - You study a gene known to be important in the...Ch. 17 - What is the basis of separation of different DNA...Ch. 17 - Prob. 3UCh. 17 - FISH analysis of a breast tumor biopsy for HER2...Ch. 17 - In terms of studying gene function, what is the...Ch. 17 - The Ti plasmid of Agrobacterium usually induces...Ch. 17 - Prob. 1ACh. 17 - Which of the following statements is accurate for...Ch. 17 - Prob. 3ACh. 17 - Many human proteins, such as hemoglobin, are only...Ch. 17 - Amyloid beta is a proteolytic product of a protein...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
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- a. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid. b. What are restriction length polymorphisms, and how are they used?arrow_forwardinvolved in the transfer of multiple drug resistance from one cell to another? * a.Transposition b. Conjugation with a cell with chromosomal drug resistance appears in the genome of a bacteriophage that has infected it. c. Transformation of chromosomal genes d. Conjugation with a cell with a free plasmid carrying drug resistancearrow_forwardA student wants to observe how the environment of bacteria affects gene expression. How should the experiment be designed? A. exposing the same species of bacteria to the same carbohydrates in each sample B. exposing the same species of bacteria to different carbohydrates in each sample C. exposing different species of bacteria to the same carbohydrates in each sample D. exposing different species of bacteria to different carbohydrates in each samplearrow_forward
- What are transgenic plants?Give some example.arrow_forwardPart A: During cloning, the DNA is cut with a restriction enzyme giving it what? A. more introns B. sticky ends C. a polyA tail D. a binding site for a transcription factor Part B: If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use? A. PCR B. epigenetics C. hybridization D. gel electrophoresis Part C: If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible? A. transcription factor B. rRNA C. ribosome D. RNA polymerase Part D: If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what? A. PCR B. gene therapy C. stem cell work D. DNA fingerprintingarrow_forwardSecond part asks: Explain the roles two of those enzymes would play in obtaining the recombinant plasmidsarrow_forward
- Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid.What are restriction length polymorphisms, and how are they used?arrow_forwardWhich of the following is an outcome that could be produced by the application of the biotechnology as described? A. using PCR to modify a crop plant for higher yields B. introducing a recombinant DNA plasmid into a cell to trigger RNAi C. identifying SNPs to match birth parents to an adopted child D. using RT-PCR to generate copies of a gene to be expressed for insulin production An SNP is found associated with individuals who have diabetes. Which statement is true? A. All individuals with the SNP will have diabetes. B. The SNP codes for a faulty protein that causes the individual to have diabetes. C. To identify the SNP in more individuals, they will need to have their genome sequenced. D. The SNP may be linked to a gene that contributes to diabetes.arrow_forwardWhat is a cloning vector? A. The DNA probe used to locate a particular gene in the genome. B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell. C. The laboratory apparatus used to clone genes. D. An enzyme that cuts DNA into restriction fragments.arrow_forward
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