Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Textbook Question
Chapter 17.1, Problem 4MI
What would you conclude if you obtained only blue colonies after cloning into a vector that enables blue/white screening of transformants? Why might such a result occur?
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Chapter 17 Solutions
Prescott's Microbiology
Ch. 17.1 - Examine the uncut piece of DNA shown in the upper...Ch. 17.1 - Which of the above enzymes yield blunt ends? Which...Ch. 17.1 - Prob. 3MICh. 17.1 - What would you conclude if you obtained only blue...Ch. 17.1 - Why must introns be removed from eukaryotic DNA...Ch. 17.1 - Which plasmid is a shuttle vector? Why?Ch. 17.1 - In what ways does the BAC shown here differ from...Ch. 17.1 - Describe restriction enzymes, sticky ends, and...Ch. 17.1 - What is cDNA? Why is it necessary to generate cDNA...Ch. 17.1 - Prob. 3CC
Ch. 17.1 - Prob. 4CCCh. 17.1 - Prob. 5CCCh. 17.2 - Why, after three cycles, are the vast majority of...Ch. 17.2 - Briefly describe the polymerase chain reaction....Ch. 17.2 - Why is PCR used to detect infectious agents that...Ch. 17.2 - How would you use PCR to measure the concentration...Ch. 17.2 - Why is it possible to visualize a PCR product on...Ch. 17.2 - Prob. 5CCCh. 17.3 - Why are long fragments (e.g., 20,000 bp) of...Ch. 17.4 - What special considerations are necessary if one...Ch. 17.4 - Prob. 1CCCh. 17.4 - Prob. 2CCCh. 17.4 - Prob. 3CCCh. 17.4 - You are studying chemotaxis proteins in a newly...Ch. 17.5 - Prob. 1MICh. 17.5 - Prob. 1CCCh. 17.5 - Prob. 2CCCh. 17 - Which of the DNA molecules shown are recombinant?Ch. 17 - Prob. 1RCCh. 17 - Prob. 2RCCh. 17 - Prob. 3RCCh. 17 - Prob. 4RCCh. 17 - Prob. 5RCCh. 17 - Prob. 6RCCh. 17 - Prob. 1ALCh. 17 - Prob. 2ALCh. 17 - Suppose you transformed a plasmid vector carrying...Ch. 17 - You are interested in the activity and regulation...Ch. 17 - Prob. 5ALCh. 17 - Prob. 6ALCh. 17 - Prob. 7AL
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Why can we use temperature sensitive genetic screening to identify particular mutants within a sample?arrow_forwardin the cloning vector, what would be the plausible impacts if a mutation at the Ori site renders it non- functional?arrow_forwardIn DNA cloning, why do we need to check colonies for insert after transformation?arrow_forward
- You want to clone a 6,000 bp DNA fragment in E. coli. Which cloning vectors would be appropriate? How will you select transformants?arrow_forwardWhat would happen if a plasmid without a selectable marker was chosen as a cloning vector?arrow_forwardOutline a PCR-based approach to screen for colonies transformed with recombinant expression plasmid that can be used for protein expression.arrow_forward
- For each of the following experimental goals, is PCR orgene cloning preferable and why?a. Isolate the same gene from 20 individuals.b. Isolate 100 genes from the same individual.c. Isolate a mouse gene when you have a rat genefragment.arrow_forwardThe temperature at which the primers and target DNA hybridize may be changed to influence the stringency of PCR amplification. What effect will changing the hybridization temperature have on the amplification? Let's say you have a certain yeast gene A and want to check whether it has a human equivalent. How might managing the hybridization's rigor benefit you?arrow_forwardIf you are using a restriction enzyme that make 8 cuts in the Lambda phage DNA, how many bands (pieces) do you expect to be generated assuming each band is a unique size?arrow_forward
- What is the possible cause of the presence of an extra band in the results of DNA extraction and PCR amplification of the ITS and Beta actin markers during agarose gel electrophoresis?arrow_forwardCloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardGive three important characteristics of cloning vectors.arrow_forward
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