Concept explainers
To determine: The effect on the cells that are treated with an agent which caused the removal of cap from messenger RNAs (mRNAs).
Concept introduction:
After transcription, pre-mRNA is synthesized and further modified before translation in the cytoplasm. This is called the RNA processing and it includes the modification of mRNA ends and RNA splicing. A modified guanine (7-methyl guanine) group is added to the 5′ end of the mRNA chain with the help of the capping enzymes. This is known as the 5′ cap. At the 3′ end of the eukaryotic mRNA chain, adenine groups (poly-A tail) are added by poly (A) polymerase enzyme. This is known as the polyadenylation.
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