Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
expand_more
expand_more
format_list_bulleted
Question
Chapter 18.2, Problem 1R
Summary Introduction
To review:
The comparison of green fluorescent protein (GFP) as a reporter gene and antibiotic resistance gene with respect to detection of recombinant deoxyribonucleic acid (DNA) in host cells.
Introduction:
In recombinant technology, to know, which host cells contain recombinant DNA, a method of detection is needed. The problem is solved by reporter genes. Their expression is easy to visualize and hence they help to detect the host cells with recombinant DNA. The most common of these reporter genes is GFP. Another most common method is the use of selectable markers like antibiotic resistance gene.
Expert Solution & Answer
Trending nowThis is a popular solution!
Students have asked these similar questions
Provide one example of a CRISPR-Cas application forbiotechnology.
Define CRISPR interference, describe its natural mechanism in bacterial defense and how it isexploited today in DNA editing.
describe how the bacteria should have the ability to be recovered back from the infected host for the purpose of experimentation including sequencing ,vitro culturing and polymerase chain reaction amplification.
Chapter 18 Solutions
Life: The Science of Biology
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Compare and contrast the advantages and disadvantages of production of genetically modified crps.arrow_forwardDescribe the role of restriction enzymes in the process of transformation.arrow_forwardWould it be appropriate to use DNA probes such as VNTR in DNA fingerprinting of an bacteriophage?arrow_forward
- Explain how the presence of an antibiotic resistance gene can be used to determine whether a transformation has been successful.arrow_forwardBriefly describe what CRISPR technology doesarrow_forwardCompare and discuss the key features of plasmids that are respectively used as bacterial cloning and bacterial expression vectors.arrow_forward
- In the bacterial transformation experiment, what is the primary purpose of using an ampicillin-containing medium? to select for transformed cells to select for wildtype cells to prevent contamination to stimulate a color changearrow_forwardInclude references! How can we analyze the interest protein expression in cultured cells after we transfect with the following vectors including gene of interest: Green fluorescent protein (GFP) including vectors His-tag including vectorsarrow_forwardDirect modification of genes may be achieved using what? (Choose one below): DNA microarray CRISPR-Cas system Hybridizationarrow_forward
- You have constructed a cDNA library in a phagemid vector.Describe how you would screen the library for a particulargene of interest. Describe methods using oligonucleotideand antibody probes.arrow_forwardDescribe two methods for transforming a vector into bacteria.arrow_forwardyou transform bacteria with the pGLO plasmid. What do you need to add to the media to get them to express the GFP gene in a pGLO transformation lab?arrow_forward
arrow_back_ios
arrow_forward_ios
Recommended textbooks for you
Serology 101: Testing for IgG and IgM antibodies; Author: Beckman Coulter Dx;https://www.youtube.com/watch?v=LtqKB-qpJrs;License: Standard youtube license