Life: The Science of Biology
Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 18.3, Problem 4R
Summary Introduction

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The reason behind a complementary deoxyribonucleic acid (cDNA) library constructed from human brain tissue being both similar to and different from a cDNA library constructed from the human pancreatic tissue.

Introduction:

A collection of different cloned deoxyribonucleic acid (DNA) sequences from an organism is called a gene library. There are usually two types of gene library, namely: genomic library and complementary DNA (cDNA) library, which depends on the source of DNA used. A collection of clones containing cDNA molecules that are usually synthesized from messenger ribonucleic acid (mRNA) molecules in a cell of an organism is called cDNA library. It contains only the coding regions of a genomic material of an organism, unlike genomic library which contains the whole genome of an organism.

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True or False; explain  It is possible that the coding region of a gene is present in a genomic library produced from a particular tissue, but is absent in cDNA library prepared from the same tissue.
What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.
. Explain why a long open reading frame suggests theexistence of a protein-coding exon
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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License