Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 18.4, Problem 3R
Summary Introduction
To review:
Both CRISPR (clustered regularly interspaced short palindromic repeat) technology and antisense ribonucleic acid (RNA) technologies are used in blocking the gene expression. Determine the advantage of using CRISPR technology over antisense RNA technology,
Introduction:
Change in deoxyribonucleic acid (DNA) sequences or any mutation causes an alteration in the proteins. The cause-and-effect relationship in proteins can be demonstrated by mutation. Alteration of protein can be stopped by inactivating specific genes. It can be done using antisense RNA and CRISPR technology.
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Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?
Gene expression can be disrupted by techniques such as homologous recombination and RNA interference. What is the functional difference between these two methods in terms of the ultimate effect on gene expression? Why might homologous recombination or an alternative genome-editing method (e.g., CRISPR/Cas9) be preferred over RNA interference?
What are the major components of the CRISPR-Cas9 system? What mechanism does it employ to combine DNA? Explain the process of how the CRISP-Cas9 system is able to create recombinant DNA. Relate the idea of gene modification to the fields of vaccines and applied microbiology as well.
Chapter 18 Solutions
Life: The Science of Biology
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- what are the advantages and disadvantages of CRISPR cas9 technology?arrow_forwardWhat is the function of the CRISPR/Cas system? What are the ethical impacts of a technology such as CRISPR?arrow_forwardConsider the following human genetic diseases: hemophilia, Down syndrome, cystic fibrosis, and brain cancer. Which are the best candidates for treatment with CRISPR-Cas genome editing, and which have the largest hurdles to overcome? Why?arrow_forward
- Some expression plasmids have small peptide or protein tags in front of and/or after the cloning site. What is the advantage of these vectors?arrow_forwardWhy is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or transgenic animals?Explain your answers.arrow_forwardwhat are the mechanism of CRISPR-Cas-9 to combine DNA? explain.arrow_forward
- Explain How Single-nucleotide mutations can be introduced into the genome using an engineered CRISPR-Cas9 system.arrow_forwardWhat are some ways that CRISPR-Cas systems have been enhanced and modified to provide additional functions?arrow_forwardWhat are the ethical implications of CRISPR cas9 technology or gene editing?arrow_forward
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