Life: The Science of Biology
Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 18.4, Problem 3R
Summary Introduction

To review:

Both CRISPR (clustered regularly interspaced short palindromic repeat) technology and antisense ribonucleic acid (RNA) technologies are used in blocking the gene expression. Determine the advantage of using CRISPR technology over antisense RNA technology,

Introduction:

Change in deoxyribonucleic acid (DNA) sequences or any mutation causes an alteration in the proteins. The cause-and-effect relationship in proteins can be demonstrated by mutation. Alteration of protein can be stopped by inactivating specific genes. It can be done using antisense RNA and CRISPR technology.

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Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?
Gene expression can be disrupted by techniques such as homologous recombination and RNA interference. What is the functional difference between these two methods in terms of the ultimate effect on gene expression? Why might homologous recombination or an alternative genome-editing method (e.g., CRISPR/Cas9) be preferred over RNA interference?
What are the major components of the CRISPR-Cas9 system? What mechanism does it employ to combine DNA? Explain the process of how the CRISP-Cas9 system is able to create recombinant DNA. Relate the idea of gene modification to the fields of vaccines and applied microbiology as well.
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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License