The effectiveness of the enhancers associated with the dark or blond hair in driving the expression of the Kitl mRNA (messenger ribonucleic acid). Introduction: KITLG gene is controlled by an enhancer and it also expresses itself for hair color in humans apart from other phenotypic traits. The signaling proteins that bind to the cell-surface receptor are coded by this gene in the cell. In the given experiment, the Kitl gene (mouse equivalent of KITLG gene) and its core promoter were bonded together by genetic engineering. The amount of Kitl mRNA was measured in the skin after its introduction in the embryo of a mouse. The results are shown in the graph provided below. Probability is given as P < 0.05 and &P **lt; 0.001.

Question

Want to see the full answer?

Answer 1

Question

Chapter 19, Problem 14PIAT

Summary Introduction

To review:

The effectiveness of the enhancers associated with the dark or blond hair in driving the expression of the Kitl mRNA (messenger ribonucleic acid).

Introduction:

KITLG gene is controlled by an enhancer and it also expresses itself for hair color in humans apart from other phenotypic traits. The signaling proteins that bind to the cell-surface receptor are coded by this gene in the cell.

In the given experiment, the Kitl gene (mouse equivalent of KITLG gene) and its core promoter were bonded together by genetic engineering. The amount of Kitl mRNA was measured in the skin after its introduction in the embryo of a mouse. The results are shown in the graph provided below. Probability is given as P < 0.05 and &P **lt; 0.001.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

E27. A cloned gene fragment contains a regulatory element that is recog- nized by a regulatory transcription factor. Previous experiments have shown that the presence of a hormone results in transcriptional acti- vation by this transcription factor. To study this effect, you conduct a electrophoretic mobility shift assay and obtain the following results: Tube: 1 2 3 Transcription factor: Hormone: Explain the action of the hormone.

Describe the mechanisms in which DNA is used to generate protein. Reflect on the key points in the process and mention any major differences between the mechanism in prokaryotic and eukaryotic cells. Although the DNA in our genes is considered to be the heritable genetic material, other factors, including the environment are considered to play an important role in the activity and expression of those genes. Summarize the role that epigenetics & developmental epigenetics play in health & disease.

E30. An electrophoretic mobility shift assay can be used to study the binding of proteins to a segment of DNA. In the experiment shown here, an EMSA was used to examine the requirements for the bind- ing of RNA polymerase II (from eukaryotic cells) to the promoter of a protein-encoding gene. The assembly of general transcription factors and RNA polymerase II at the core promoter is described in Chapter 12 (Figure 12.14). In this experiment, the segment of DNA containing a promoter sequence was 1100 bp in length. The fragment was mixed with various combinations of proteins and then subjected to an EMSA. Lane 1: No proteins added Lane 2: TFID Lane 3: TFIIB Lane 4: RNA polymerase I| Lane 5: TFID + TFIIB Lane 6: TFID + RNA 1 2 4 5 6 polymerase II| Lane 7: TFIID + TFIIB + RNA polymerase I| 1100 bp Explain which proteins (TFIID, TFIIB, or RNA polymerase II) are able to bind to this DNA fragment by themselves. Which transcrip- tion factors (i.e., TFIID or TFIIB) are needed for the binding of…