Interpretation:
The graph which represents the activity curve of the reaction in which EDTA is added to a reaction mediated by EcoRI has to be determined.
Concept Introduction:
Enzyme inhibitors: The substance which slows or stops the action of an enzyme is called enzyme inhibitors.
It can be used as drugs and an example is AZT which is used to treat HIV.
The competition of an enzyme can be reversible or irreversible. In reversible inhibition, the inhibitor can leave and in irreversible inhibition, the inhibitor remains permanently bound.
Reversible Competitive inhibition: It is a type of inhibition occurs when the inhibitor resembles very much to the substrate and thus prevents the substrate binding.
Irreversible competitive inhibition: It is a type of inhibition in which an inhibitor forms covalent bonds to the active site and thereby permanently blocking it.
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Fundamentals of General, Organic, and Biological Chemistry (8th Edition)
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- The enzyme, fumarate, has the following kinetic constants: k 1 k 2 k -1 where k 1 = 10 9 M -1 s -1 k -1 =4.4 x 10 4 s -1 k 2 = 10 3 s -1 a. What is the value of the Michaelis constant for this enzyme? b. At an enzyme concentration of 10 -6 M, what will be the initial rate of product"arrow_forwardWould you expect an “enzyme” designed to bind its target substrate as tightly as it binds the reaction transition state to show a rate enhancement over the uncatalyzed reaction? In other words, would such protein be a catalyst? Use a reaction energy diagram to explain why or why not.arrow_forwardWhat are the three key mechanisms and their associated features that are utilized by enzymes to catalyze reactions. Additionally, which two mechanims of the three you listed does the enzyme chymotrypsin use?arrow_forward
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