Concept explainers
(a)
To explain: The step in polypeptide synthesis inhibited by Rambomycin.
Introduction: The polypeptide chain refers to the sequence of amino acids which are linked with the help of peptide bonds. The process of polypeptide synthesis refers to the generation of peptides. The polypeptide synthesis is carried out with the help of translation and transcription.
(b)
To determine: Whether the oligonucleotide product be attached to tRNA at the end of the uninhibited reaction and will the dipeptide product be attached to tRNA at the end of Rambomycin-inhibited reaction.
Introduction: The polypeptide chain refers to the sequence of amino acids which are linked with the help of peptide bonds. The process of polypeptide synthesis refers to the generation of peptides. The polypeptide synthesis is carried out with the help of translation and transcription.
Want to see the full answer?
Check out a sample textbook solutionChapter 19 Solutions
WORLD OF CELL+MASTERING ACCESS >CUSTOM
- Question:- Describe the function of each of the following Shortly. a. Amino-acyl tRNA synthetase b. E coll release factors 1 and 2 (RF1 and RF2) c. 5' methyl-guanosine cap d. Ribosomal P sitearrow_forwardCentral Dogma of Molecular Biology from DNA to RNA to Protein, discussing the principles underlying the transfer of information in a biologic system and its regulation. However, recent research seems to challenge certain aspects of Crick’s Central Dogma. Does the Central Dogma still stand today? If not, can you find an example for a type of information transfer that is not explicitly covered by the Central Dogma (or even violates it)?arrow_forwardProblem: From the following information determine the amino acid sequence of a peptide. N-terminal Edman gives PTH-Alanine C terminal carboxypeptidase treatment, no observable reaction Trypsin cleavage gives three products Arg Peptide containing Ala, Lys Peptide containing Asp, Met, Phe, Pro Mild Chymotrypsin cleavage gives 2 peptides Peptide containing Asp, Pro Peptide containing Ala, Arg, Lys, Met, Phe CNBr cleavage gives 2 peptides Peptide containing Ala, Arg, Lys and homoserine Peptide containing Asp, Phe, Pro You must supply the answer as the 3-letter amino acid sequence from N-terminus to C-terminus in the form (you must use dashes, not spaces between the amino acids) Met-Thr-Glu-Trparrow_forward
- disadvantage of using protein blastarrow_forwardYes or no? Is sequence of riboprobe identical to the mrna produced by gene in situ hybridization? does column of purification in DNA allow it to flow while other molecules are trapped ?arrow_forwardFast please At 20 Celsius degree, protein-Z binds DNA with a free energy of –7 kJ/mole. Protein Z has an unfolding free energy of 11 kJ/mole without ligand bound and 15 kJ/mole with ligand bound. Does the unfolded/denatured form of protein Z bind to DNA, and if so – what is the "Delta G" of binding of the unfolded form?arrow_forward
- if the reaction pH drops significantly (pH=2 for example), how would reaction catalyzed be serine proteases be affected? why? (assumin that the rest of the protein structure remain intact)arrow_forwardBIOMOLECULES - MULTIPLE CHOICE - Please answer properly QUESTION : If a cell has an adequate supply of adenine nucleotides but requires more guanine nucleotides for protein synthesis: 1. Glutamine-PRPP amidotransferase will not be fully inhibited 2. AMP will be a feedback inhibitor of the condensation of IMP with aspartate 3. ATP will stimulate the production of GMP from IMP 4. ATP will inhibit nucleoside diphosphate reductase. Choices A. 2 and 4 B. 1 and 3 C. 1, 2, 3, and 4 D. 1, 2, and 3arrow_forwardSuggest a reasonable strategy for the specific phosphorylation of the5’ –OH group of a nucleoside.arrow_forward
- Hypothesis: Glycans terminated in sialic acid monosaccharides may be necessary to maintain embryonic stem cells in their undifferentiated state. Imagine that you are the scientist trying to prove this hypothesis with skill genetic engineering. What classes of enzymes would you target for elimination to generate embryonic stem cells that do not carry sialic acid modifications to evaluate this hypothesis?arrow_forwardHelp? Please explain salting out in protein purification.arrow_forwardBIOLOGY ACTIVITY -Gene Mutations and Proteins Objective: To demonstrate how gene mutations affect the production of proteins? Procedure: Use the following base sequence of one strand of an imaginary DNA molecule: AATTGAACACATGCGCCC. 2. Write the base sequence for an mRNA strand that would be transcribed from the given DNA sequence. Place your results in the table below. Use your codon table provided below to determine the sequence of amino acids in the resulting protein fragment. Place your results in the table below. If the fifth base in the original DNA strand were changed from G to C, how would this affect the resulting protein fragment? Write the new protein fragment in the table below. If G were added to the original DNA strand after the third base, what would the resulting mRNA look like? How would this addition affect the protein? Show your results in the table below. Data: mRNA from Step 2 Protein Sequence from Step 3 Protein Sequence from Step…arrow_forward
- Biology: The Unity and Diversity of Life (MindTap...BiologyISBN:9781305073951Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa StarrPublisher:Cengage LearningBiology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage LearningBiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning