Biological Science (6th Edition)
6th Edition
ISBN: 9780321976499
Author: Scott Freeman, Kim Quillin, Lizabeth Allison, Michael Black, Emily Taylor, Greg Podgorski, Jeff Carmichael
Publisher: PEARSON
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Chapter 19, Problem 2TYK
Summary Introduction
Introduction:
Enhancers are a short sequence of the DNA (deoxyribonucleic acid) that are capable of binding to the proteins in order to elevate the occurrence of the transcription process of a specific gene. These proteins are also known as activators and are generally referred to as transcription factors.
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a) What is a gene promoter? b) What proteins bind to a promoter? c) What do those proteins do to control gene expression?
An enhancer, located upstream from a gene, has the following sequence:
5′–GTAG–3′ 3′–CATC–5′
This enhancer is orientation-independent. Which of the following sequences also works as an enhancer?
A. 5′–CTAC–3′ 3′–GATG–5′
B. 5′–GATG–3′ 3′–CTAC–5′
C. 5′–CATC–3′ 3′–GTAG–5′ C15.
What strategy does a genetically encoded calcium indicator look like to allow fluorescence imaging of only one cell type in an acute slice of the brain?
A.The use of fluorescent protein expression inhibitors in other cells
B.The injection of a recombinant virus causing the death of other cells
C.The use of a promoter specific to these cells
D.Activation of membrane receptors specific to these cells
Chapter 19 Solutions
Biological Science (6th Edition)
Ch. 19 - What is chromatin? a. the histone-containing...Ch. 19 - Prob. 2TYKCh. 19 - Prob. 3TYKCh. 19 - Prob. 4TYKCh. 19 - Compare and contrast the items in each pair: (a)...Ch. 19 - Prob. 6TYUCh. 19 - Prob. 7TYUCh. 19 - Prob. 8TYUCh. 19 - Prob. 9TYPSSCh. 19 - 10. QUANTITATIVE Imagine repeating the experiment...
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- You are curious to identify the region of the gene X sequence that serves as an enhancer for gene expression. Design an experiment to investigate this issue.arrow_forwardYou are studying a new gene that is expressed within the adipose tissue of humans during the development of diabetes. Specifically, you want to learn why gene expression levels are very high in those people who develop diabetes. What DNA sequence would you concentrate on? Group of answer choices A specific response elements B the TATA box C enhancer sequences D promoter proximal elements E metal response elementsarrow_forwardAn enhancer is surrounded by four genes (A, B, C, and D), as shown in the accompanying diagram. An insulator lies between gene C and gene D. On the basis of the positions of the genes, the enhancer, and the insulator, the transcription of which genes is most likely to be stimulated by the enhancer? Explain your reasoning.arrow_forward
- A certain Drosophila protein-encoding gene has one intron. If a large sample of null alleles of this gene is examined, will any of the mutant sites be expecteda. in the exons?b. in the intron?c. in the promoter?d. in the intron–exon boundary?arrow_forwardWhich of the following mutations could be appropriately describedas a position effect?A. A point mutation at the –10 position in the promoter regionprevents transcription. B. A translocation places the coding sequence for a muscle-specificgene next to an enhancer that is turned on in nerve cells.C. An inversion flips a gene from the long arm of chromosome 17(which is euchromatic) to the short arm (which isheterochromatic).arrow_forwardYour investors are concerned that the GasP protein might not be sufficiently produced under normal laboratory conditions. They suggest controlling the transcription of the gasP gene using a chemical that will “trigger” its transcription. a. What type of promoter could be used? b. What chemical will you use to control transcription? c. How does this method of control work?arrow_forward
- If a mutation occurred in a control element sequence associated with a particular gene, which of the following would be a likely consequence of this? A. This mutation is not likely to affect the expression of the associated gene, because RNA polymerase does not bind to control elements. B. This will likely affect the expression of the associated gene because it will loosen the coiling of chromatin at this site. C. The expression of the associated gene will be affected because this change will result in a nonsense mutation. D. This mutation is not likely to affect the expression of the associated gene, since control elements are noncoding sequences. E. The associated gene's expression would likely be affected, since this mutation would change the ability of at least one transcription factor to bind.arrow_forwardWhat is the difference between a promoter and an enhancer?arrow_forwardWhich of the following is true of CpG islands? a. They are methylated near promoters of actively transcribed genes. b. They are unmethylated near promoters of actively transcribed genes. c. Acetylation of CpG islands leads to repression of transcription. d. CpG islands code for RNA molecules that activate transcription.arrow_forward
- a. Some antibiotics, such as rifampin, interfere with the function of RNA polymerase. What biological process is rifampin disrupting? b. Some antibiotic-resistant M. tuberculosis bacteria have a single point mutation (CàT) in the rpoB gene that causes an amino acid change from serine (a polar amino acid) to leucine (a non-polar amino acid). What type of mutation is this? Do you expect this to have no effect, a small effect, or a large effect on the polypeptide produced? Explain your reasoning. c. The rpoB gene encodes a subunit of the bacterial RNA polymerase protein. The point mutation described in Question 2 causes a change in protein folding, which leads to the inability of the rifampin antibiotic to bind to the RNA polymerase. Which level(s) of protein structure is/are affected by this change?arrow_forwardWhat biomolecule are promoters and enhancers composed of?arrow_forwardWhat are differences between promoters and enhancers ?arrow_forward
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