Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 2, Problem 24P
Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pKa = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

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