Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 2, Problem 20P

A biochemical reaction takes place in a 1.00 ml solution of 0.0250 M phosphate buffer initially at pH = 7.20 (see Table 2.6 for pkas of phosphate species).
a. Are the concentrations of any of the four possible phosphate species negligible? If so, identify them and explain your answer.
b. During the reaction, 3.80 µmol of HCI are produced. Calculate the final pH of the reaction solution. Assume that the HCI is completely neutralized by the buffer.

Chapter 2, Problem 20P, A biochemical reaction takes place in a 1.00 ml solution of 0.0250 M phosphate buffer initially at

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