Human Biology: Concepts and Current Issues (8th Edition)
8th Edition
ISBN: 9780134312729
Author: Johnson
Publisher: PEARSON
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Chapter 20, Problem 10TY
Summary Introduction
Introduction:
The transfer of pieces of DNA (deoxyribonucleic acid) from one organism into another is made possible through recombinant DNA technology. In this process, specific genes are inserted into the bacteria so that it can be induced to produce useful proteins. It requires specialized tools and components, which includes restriction enzymes, DNA ligases, plasmids, and bacteria.
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The function of a restriction enzyme is to
a. prevent the movement of DNA outside the nucleus
b. separate the DNA double helix
c. cut the nucleotide sequence at a specific location in DNA
d. proofread DNA for accidental damages and corrects these errors
What is the enzymatic function of restriction enzymes?
Group of answer choices
a. to cut nucleic acids at specific sites
b. to join nucleotides during transcription
c. to add new nucleotides to the growing strand of DNA
d. to repair breaks in sugar - phosphate backbones
separates molecules by movement due to size and electrical charge
[ Choose ]
a. DNA ligase
b. gene cloning
c. gel electrophoresis
d. restriction enzymes
Chapter 20 Solutions
Human Biology: Concepts and Current Issues (8th Edition)
Ch. 20 - Prob. 1QCCh. 20 - GM foods are created for a variety of reasons,...Ch. 20 - Should foods that contain genetically modified...Ch. 20 - Explain how DNA is sequenced.Ch. 20 -
2. Describe the enzymes used in recombinant DNA...Ch. 20 - Prob. 3CRCh. 20 - Discuss the polymerase chain reaction technique,...Ch. 20 - Prob. 5CRCh. 20 - Prob. 6CRCh. 20 - Prob. 7CR
Ch. 20 - Prob. 8CRCh. 20 - Prob. 9CRCh. 20 -
10. List the diseases that gene therapy may be...Ch. 20 - Prob. 1TYCh. 20 - Prob. 2TYCh. 20 - Which of the following is used to separate...Ch. 20 - Genetically engineered human insulin, human growth...Ch. 20 - Prob. 5TYCh. 20 - All of the following statements about transgenic...Ch. 20 -
7. "Gene pharming” refers to:
a. using transgenic...Ch. 20 - Prob. 8TYCh. 20 - Prob. 9TYCh. 20 - Prob. 10TYCh. 20 - Which of the following is needed for sequencing...Ch. 20 - A tiny amount of blood was recovered from a crime...Ch. 20 - Which technique will allow amplification of the...Ch. 20 - A gene for a bacterial protein with insecticide...Ch. 20 - Prob. 15TYCh. 20 -
1. What do you think are the chances that someone...Ch. 20 - Imagine a future in which the respiratory effects...Ch. 20 - Prob. 3AWKCh. 20 - Prob. 4AWKCh. 20 - Prob. 5AWKCh. 20 - There is interest in producing vaccines in foods....Ch. 20 - Transgenic plants have the potential to improve...
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- Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerasearrow_forwarda) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c) Compare and contrast the these enzymes in nature and in scientific research.arrow_forwardThe activity of restriction enzymes may produce fragments with sticky ends. Sticky ends are a) a type of endonucleases. b) dephosphorylated CpG islands. c) unpaired nucleotides. d) double breaks with blunt ends.arrow_forward
- Which of the following is a function of a restriction endonuclease? a. It cleaves a DNA molecule at a specific site. b. It cleaves a DNA molecule at random locations. c. It removes specific codons from an mRNA molecule. d. It removes specific exons from an mRNA molecule.arrow_forwardWhy is DNA ligase so important in recombinant DNA technology? a.) It causes DNA to make multiple copies of itself. b.) It joins two DNA fragments together. c.) It shapes bacterial DNA into a circular plasmid. d.) It cuts DNA into restriction fragments.arrow_forwardExplain how recombinant DNA technology uses restriction enzymes (restriction endonucleases). Explain how recombinant DNA technology uses DNA ligase enzymes.arrow_forward
- Which gene is incorporated into plasmids to detect recombinantcells?a. restriction endonuclease b. virus receptors c. a gene for antibiotic resistance d. reverse transcriptasearrow_forwardWhat is a cloning vector? A. The DNA probe used to locate a particular gene in the genome. B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell. C. The laboratory apparatus used to clone genes. D. An enzyme that cuts DNA into restriction fragments.arrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forward
- A contig is a. a set of molecular markers used in gene mapping. b. a set of overlapping fragments that form a continuous stretch of DNA. c. a set of fragments generated by a restriction enzyme. d. a small DNA fragment used in sequencing.arrow_forwardWhat do genetic engineers use to create the “sticky ends” needed to splice two fragments of DNA together? a.) an amino acid sequence b.) DNA ligase c.) restriction enzymes d.) mRNAarrow_forwardMake a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it producesarrow_forward
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