Campbell Biology: Custom Edition
Campbell Biology: Custom Edition
18th Edition
ISBN: 9781323717271
Author: Urry, Cain, Wasserman, Minorsky, Reece
Publisher: PEARSON C
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Chapter 20.1, Problem 1CC
Summary Introduction

To determine: The bonds cleaved when restriction enzyme PvuI cuts its specific site in the DNA sequence.

Concept introduction:

Restriction enzymes recognize their specific target sequence and excise DNA at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. These make staggered cuts at or adjacent to their specific recognition site and most of them produce sticky ends.

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3 The restriction endonuclease Pstl cuts DNA symmetrically on both strands at the СТGCAG sequence: GACGTC On the resulting DNA fragments are left 3' overhanging ends of 4 nucleotides. PstI cleaves the phosphodiester backbone with a-type specificity. In short-hand notation, showing the identity of phosphates on the ends, draw the two fragments that result from PstI digestion of the following double-stranded oligonucleotide: 5'-рAATTGCTAСTGCAGAACCGG-3' 3'-ТТAАCGATGACGTCTTGGСС--5'
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