GENETICS:ANALYSIS+PRIN.(LL)-W/ACCESS
6th Edition
ISBN: 9781260239775
Author: BROOKER
Publisher: MCG
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Chapter 21, Problem 20EQ
What is the purpose of a Northern blotting experiment? What types of information can it tell you about the transcription of a gene?
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Describes a method known as Western blotting that can be used to detect a polypeptide that is translated from a particular mRNA. In this method, a particular polypeptide or protein is detected by an antibody that specifically recognizes a segment of its amino acid sequence. After the antibody binds to the polypeptide within a gel, a secondary antibody (which is labeled) is used to visualize the polypeptide as a dark band.For example, an antibody that recognizes α-galactosidase A couldbe used to specifically detect the amount of α-galactosidase A proteinon a gel. The enzyme α-galactosidase A is defective in individuals with Fabry disease, which shows an X-linked recessive pattern of inheritance. Amy, Nan, and Pete are siblings, and Pete has Fabry disease. Aileen, Jason, and Jerry are brothers and sister, and Jerry has Fabry disease. Amy, Nan, and Pete are not related to Aileen, Jason, and Jerry. Amy, Nan, and Aileen are concerned that they could be carriers of a defective…
Chapter 21 Solutions
GENETICS:ANALYSIS+PRIN.(LL)-W/ACCESS
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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- Northern blots are valuable tools to analyze the mRNA level present in a sample. Describe an experiment that uses the Northern blot as a tool and how you would visualize the mRNA giving at least two examples of probe types.arrow_forwardWhat is alternative splicing? In which organisms can this be detected? Is it an advantage or disadvantage for the organism? Briefly explainarrow_forwardWhat do transposable genes mean? How many different transposable genes do bacteria contain?arrow_forward
- What factors do we consider in genetic transcription: precision, accuracy, or a combination of the two? What happens to the genetic coding if it encounters an error during the process of forming. Cite a few instances where coding was thrown off track.arrow_forwardIn chapter 19 of Watson et al., Box 19-2 explains the ChIP-Chip and ChIP-Seq techniques. Explain how can these techniques result in specific evidence for the combinatorial control of a eukaryotic gene?arrow_forwardThere is a hypothetical gene related to the nervous system of Drosophila. Describe all the methods, steps, and key substances you need to obtain to use the following techniques in experimental design to study the gene: - In situ hybridization (to find the mRNA) - Immunohistochemistry (to find the protein) - CRISPR-Cas9 (for loss of function) - Expression vector (for gain of function)arrow_forward
- What is meant by the term attenuation? Is it an example of generegulation during transcription or translation? Explain your answer.arrow_forwardExplain the difference in results for the RT-PCR from the CD4+ T cells and the RT-PCR from the CD8+T cells. (i.e., propose a conclusion)arrow_forwardWhat are the functions of transcriptional activator proteins and repressor proteins? Explain how they work at the molecular level.arrow_forward
- How can DNA microarrays be used to determine which genes of a bacterial pathogen are expressed both inside and outside of a host cell?arrow_forwardWhat is a transducing particle?arrow_forwardIn a competitive RNA microarray experiment, you hybridize two samples to the same chip. Before hybridization, sample A is labeled with red fluorescence and Sample B is labeled with green fluorescence. Spot 1142 is yellow. What does this tell you about the relative abundance of the mRNA corresponding to this spot in the two samples? Group of answer choices It is equally abundant in both samples A and B. It is more abundant in B than A. It is more abundant in A than B. It is not present in either A or B.arrow_forward
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