The results of western blot of a gene on mutation in varying conditions like: 1. Normal gene from a plant. 2. Gene without promoter. 3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position. 4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp). 5. Homozygous mutation in which phenylalanine is changed to leucine. Introduction: In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.

Question

Want to see the full answer?

Answer 1

Question

Chapter 21, Problem 25EQ

Summary Introduction

To analyze:

The results of western blot of a gene on mutation in varying conditions like:

1. Normal gene from a plant.

2. Gene without promoter.

3. Heterozygous mutation in which one gene is normal and other is short due to introduction of a stop codon at 112 position.

4. Homozygous mutation in which stop codon is inserted at 112 base pair (bp).

5. Homozygous mutation in which phenylalanine is changed to leucine.

Introduction:

In western blotting, the fragments of polypeptides that are produced within the cell are detected by using the antibodies as probes. The proteins from cell are purified and are loaded in the electrophoresis. These proteins then are observed by autoradiography or fluorescence.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

After Drosophila DNA has been treated with a restriction enzyme, the fragments are inserted into plasmids and selected as clones in E. coli. With the use of this “shotgun” technique, every DNA sequence of Drosophila in a library can be recovered.a. How would you identify a clone that contains DNA encoding the protein actin, whose amino acid sequence is known?b. How would you identify a clone encoding a specific tRNA?

(c) By binding one L-tryptophan molecule/monomer, the trp repressor binds to DNA to suppress syn- thesis of L-tryptophan in E. coli. Below is the amino acid sequence of the helix – (reverse) turn – helix region of the trp repressor that binds to DNA compared to the sequence of the corresponding DNA binding motif of the Prl protein, a different type of repressor protein. A diagram of the trp repressor dimer is also shown. reverse turn trp helix 4 70 Trp -Gly-Glu-Met-Ser-Gln-Arg-Glu-Leu-Lys-Asn-Glu-Leu-Gly-Ala-Gly- Ile- Prl -Ser-Glu-Glu-Ala-Lys-Glu-Glu-Leu-Ala-Lys-Lys-Cys-Gly-Ile-Thr- Val- Pri heilix trp helix 5 80 90 Trp Ala-Thr-Ile-Thr-Arg-Gly-Ser sgn-Ser-Leu-Lys-Ala-Ala- Prl Ser-Gln-Val-Ser-Asn-Trp-Phe-Gly-Asn-Lys-Arg-Ile-Arg- Prl helix

Knowing that the genetic code is almost universal, a scientist uses molecular biological methods to insert the human β-globin gene (Shown in Figure 17.11) into bacterial cells, hoping the cells will express it and synthesize functional β-globin protein. Instead, the protein produced is nonfunctional and is found to contain many fewer amino acids than does β-globin made by a eukaryotic cell. Explain why.