Chapter 21, Problem 33EQ

Summary Introduction

To review:

The rationale behind DNase (deoxyribonuclease) I foot printing experiment.

Introduction:

The interaction between DNA (deoxyribonucleic acid) and protein can be studied either by EMSA (electrophoretic mobility shift assay) or by DNase I foot printing technique. This technique uses DNase I enzyme for cleaving the DNA and forming several fragments, which are then put under electrophoresis.