BIOLOGY
5th Edition
ISBN: 9781264104680
Author: BROOKER
Publisher: MCG
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Chapter 21.2, Problem 2CC
Summary Introduction
To determine: The information that is provided regarding gene expression if a fluorescent spot appears on a microarray.
Introduction: Genome is defined as the complete genetic material that is present in an organism. It consists of the coding as well as the non-coding parts of DNA. Genomics is the study of the genome. DNA microarray is a technique to detect the gene expression of multiple genes at the same time.
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Genomic sequencing cannot be used to:
Predict a protein coding gene
Predict structure and function of a protein encoded in a gene
Locate similar sequences (sequence homology)
Locate repetitive sequences
Predicting alternative splicing patterns
Discuss the similarities and differences between the methods used in genomics and transcriptomics.
Both microarray and RNA-sequencing can study the transcriptomes. Compare microarray and RNA sequencing technique in analyzing gene with high, medium and low copy number of genes.
Chapter 21 Solutions
BIOLOGY
Ch. 21.1 - Prob. 1CSCh. 21.1 - Prob. 1CCCh. 21.1 - Prob. 2CCCh. 21.1 - Prob. 3CCCh. 21.2 - Prob. 1CCCh. 21.2 - Prob. 2CCCh. 21.2 - Prob. 3CCCh. 21.3 - Prob. 1EQCh. 21.3 - Prob. 2EQCh. 21.3 - Prob. 3EQ
Ch. 21.4 - Prob. 1CCCh. 21.4 - Prob. 1CSCh. 21.5 - Prob. 1CSCh. 21.5 - Repetitive Sequences and Transposable Elements...Ch. 21 - Prob. 1TYCh. 21 - DNA ligase is needed in a cloning experiment a. to...Ch. 21 - Prob. 3TYCh. 21 - Why is Taq polymerase used in PCR rather than...Ch. 21 - Lets suppose you want to clone a gene that has...Ch. 21 - In the CRISPR-Cas technology for editing genes,...Ch. 21 - Prob. 7TYCh. 21 - The enzyme that helps short segments of DNA move...Ch. 21 - Prob. 9TYCh. 21 - Which of the following was not a goal of the Human...Ch. 21 - Prob. 1CQCh. 21 - Briefly describe whether or not each of the...Ch. 21 - Prob. 3CQCh. 21 - Identify and discuss three important advances that...Ch. 21 - Prob. 2COQ
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- The technique of fluorescence in situ hybridization (FISH) is described. This is another method for examining sequence complexity within a genome. In this method, a DNA sequence, such as a particular gene sequence, can be detected within an intact chromosome by using a DNA probe that is complementary to the sequence.For example, let’s consider the β-globin gene, which isfound on human chromosome 11. A probe complementary to theβ-globin gene binds to that gene and shows up as a brightly colored spot on human chromosome 11. In this way, researchers can detectwhere the β-globin gene is located within a set of chromosomes. Becausethe β-globin gene is unique and because human cells are diploid(i.e., have two copies of each chromosome), a FISH experimentshows two bright spots per cell; the probe binds to each copy ofchromosome 11. What would you expect to see if you used thefollowing types of probes?A. A probe complementary to the Alu sequenceB. A probe complementary to a tandem array near…arrow_forwardGenetic engineering and gene therapy are similar fields within genomics. What do they have in common and what distinguishes them?arrow_forwardExaplain the two methods to study the contents of transcriptomes which are microarray analysis and RNA sequencing. Describe the goals, methodology, and outcomes for the methods that are used.arrow_forward
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