Concept explainers
(a)
Interpretation:
The order of elution of aspartate before serine by
Concept introduction:
Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.
Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.
Cation-exchange chromatography separate amino acids according to their
(b)
Interpretation:
The order of elution of serine before alanine by
Concept introduction:
Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.
Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.
Cation-exchange chromatography separate amino acids according to their
(c)
Interpretation:
The order of elution of valine before leucine by
Concept introduction:
Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.
Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.
Cation-exchange chromatography separate amino acids according to their
(d)
Interpretation:
The order of elution of tyrosine before phenylalanine by
Concept introduction:
Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.
Dowex-50 is a cation-exchange resins with less polar used for Cation-exchange chromatography.
Cation-exchange chromatography separate amino acids according to their
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Chapter 21 Solutions
Organic Chemistry (8th Edition)
- The pKa values for the two ionizable functional groups (3.39 and 8.03)differ considerably from the pKa values of alanine (2.35 and 9.87; ). Account for the observed pKa differencesarrow_forwardAn unknown amino acid labeled KEMISTRI has an IpH value equal to 5.41. If its pKa1 is equal to 2.03, calculate the pKa2 of KEMISTRI. Show complete solutions. Based on the table below, what is the probable identity of KEMISTRI?arrow_forwardGlycine solution was titrated against NaOH and pH of the final solution was 4.8. Given that the pKa¹ and pKa² of glycine are 2.34 and 9.60, respectively. Calculate the concentration of the dissociated amino acid in terms of the undissociated amino acid, if [x] and [y] are the concentrations of dissociated and undissociated amino acid, respectively.arrow_forward
- Explain the order of elution (with a buffer of pH 4) of the following pairs of amino acids through a column packed with Dowex 50 a. aspartate before serine c. valine before leucine b. serine before alanine d. tyrosine before phenylalaninearrow_forwardChemistry Given TETRAPEPTIDE: Prolinyl-Argininyl-Cysteinyl-Tryptophan Predict the direction of the peptide in electrophoresis at pH 7 and pH11.5. (Use table on the upper right for the estimated pK values of some functional groups).arrow_forwardPlease create the titration curve of aspartate and annotate the corresponding isoforms.arrow_forward
- (b) Describe how the charge of some amino groups in a protein might differ at pH 9.0 and pH 5.0. the charge on the amino group will differ at pH 5 and pH 9 which will depend on the pKa of the amino acid. (c) Describe how the charge of some carboxyl groups in a protein might differ at pH 9.0 and pH 5.0. charge on carboxyl group will differ at pH 5 and pH 9 which will depend on the pKa of the amino acid. (d) Given your answers to parts (b) and (c), what kind of intramolecular interactions in beta-galactosidase are most likely to be affected by a change in pH from 9.0 to 5.0? (e) Could the interactions you mention in part (d) affect the catalytic activity of beta-galactosidase?arrow_forwardOne of the drawbacks of older methodology for nonprotein testing was that ________________, which modern methods have corrected for. Question 2 options: A) a protein-free filtrate needed to be made B) it was difficult to separate BUN from creatinine C) ammonia was too volatile D) substrate was a limiting factorarrow_forwarda) A solution of amino acid having carboxylic side chain was titrated against NaOH. If initial pH of the solution was 2.8. Describe the state of the functional groups of the amino acid at the initial pH and at 10.5.b) Briefly explain how you will separate amino acids mixture using the charge difference between the amino acids. c) A given glycine solution was titrated against NaOH and pH of the final solution was 4.8. Given that pKa1 and pKa2 of glycine are 2.34 and 9.60, respectively. Calculate the concentration of the dissociated amino acid in terms of the undissociated amino acid, if [x] and [y] are the concentrations of dissociated and undissociated amino acid, respectively.arrow_forward
- The pKa value for adenine (N-1) is 4.2. Using the Henderson-Hasselbalch equation, calculate the base/acid ratio at pH 7. In 30 words or less, what is the significance of the predominant form at pH 7 in relation to the formation of the DNA double helix?arrow_forwardIn the case of serine, threonine and tyrosine they have the -OH group in their residue. I'm wondering even though all of them has the -OH groups in their residue. but only the tyrosine has the pKa value with the -OH grouup.arrow_forwardAccording to the paper, F486 is a phenylalanine residue (i.e., an amino acid that is part of a chain that forms a protein) on SARS-CoV-2. It has an important interaction with ACE2 (best seen in Fig. 1(C) and Fig. 2(A)). Looking at this phenylalanine and the green residues surrounding it, which statement best describes the interaction that could be occurring? a) It forms a hydrogen bond with Y83. b) It forms a dipole-dipole interaction with M82. c) It forms London dispersion forces with surrounding residues. d) It encounters repulsions from surrounding residues.arrow_forward
