Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 22, Problem 2CT
Assume that a DRT value for autoclaving a culture is 1.5 minutes. How long would it take to kill all the cells if
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Assume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution?
helpful formula:
g (generation time) = 0.301 (time)/ log x – log xo
Calculate the growth rate and generation time of a culture that increases in the exponential phase from 5x102 to1x108 cells in 12 hours
A specific heat treatment used on a particular bacterial suspension has a DRT of 10 seconds, how much time would be required to achieve a 5 log reduction in numbers of bacteria?
Chapter 22 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 22 - Prob. 1QCh. 22 - Prob. 2QCh. 22 - Give an example of a nonlaboratory use of each of...Ch. 22 - Define the term pasteurization. What is the...Ch. 22 - Explain why fungi and Bacillus sometimes grow...Ch. 22 - Assume that a DRT value for autoclaving a culture...Ch. 22 - Indicators are used in autoclaving to ensure that...Ch. 22 - A biological indicator used in autoclaving is a...Ch. 22 - The source of Legionella causing...
Additional Science Textbook Solutions
Find more solutions based on key concepts
1. Which is a function of the skeletal system? (a) support, (b) hematopoietic site, (c) storage, (d) providing ...
Anatomy & Physiology (6th Edition)
3. Trails that are derived from a common ancestor, like the bones of human arms and bird wings, are said to be_...
Biological Science (6th Edition)
In pea plants, plant height, seed shape, and seed color are governed by three independently assorting genes. Th...
Genetic Analysis: An Integrated Approach (2nd Edition)
Plants use the process of photosynthesis to convert the energy in sunlight to chemical energy in the form of su...
Campbell Essential Biology with Physiology (6th Edition)
2. List the subdivisions of the thoracic and abdominopelvic cavities.
Human Anatomy & Physiology
QWhich method, ARISA or T-RFLP, would provide more detail about microbial community complexity? Why?
Brock Biology of Microorganisms (15th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Calculate the growth rate and generation time of a culture that increases in the exponential phase from 5 x 102 to 1 x 108 cells in 12 hours.arrow_forwardAssume you have a stock culture at 5 x 109 cells/mL and you wish to inoculate 1 liter of fresh medium so that in 15 hours the cell density will be 2 x 108/mL. Assume a generation time of 3.5 hours. What should be the dilution?arrow_forwardIn autoclaving media and glassware, why do you need to maintain the temperature at 121 0C and pressure at 15 psi for 15 to 20 min? What will happen if this was done in only for 10 min? Can you autoclave lab materials and decontaminate cultures at the same time?arrow_forward
- The culture you are working has a doubling time of 2 hours and a cell density of 3 x 106 cells per mL. For your experiment, you first dilute the culture 100 fold. Assuming that there is no lab phase and that the cells remain in exponential growth the entire time, what is the cell density (cells/mL) after 10 hours?arrow_forwardWhat is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?arrow_forwardIf you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagramarrow_forward
- Given a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?arrow_forwardDuring the heat shock step, a student accidentally heated the cells at 42°C for 5 minutes. Only a few colonies were found on the agar plates at the end of the experiment. Explain the results.arrow_forwardWhat would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?arrow_forward
- Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture would you add to the new media?arrow_forwardFor an exponentially growing culture that increases from 5 *106cells/ml to 5 * 108cells/ml in 8 h, calculate g, n, and k forthis culture.arrow_forwardProvide comparison and illustration of expected growth curves from a pure culture with the following: Low initial density Actively growing cells Dormant cells Old culture measured by optical densityarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY